hprT
Register now for Subtillery 2022, the International Online Meeting on Bacillus subtilis! It will be held from the 19th-22th of September.

hprT
168

trigger enzyme, hypoxanthine phosphoribosyltransferase and part of a transcription activator

locus
BSU_00680
Molecular weight
20.10 kDa
pI
4.6
Protein length
Gene length
function
purine salvage and interconversion, control of [gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] expression
product
hypoxanthine phosphoribosyltransferase
essential
no
ec
2.4.2.8
synonyms
hprT

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0634

This gene is a member of the following regulons

Gene
Coordinates
76,344 → 76,886
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
diphosphate + IMP --> 5-phospho-α-D-ribose 1-diphosphate + hypoxanthine (according to UniProt)
diphosphate + GMP --> 5-phospho-α-D-ribose 1-diphosphate + guanine (according to UniProt)
Protein family
[wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)
Structure
[PDB|6D9Q] (apo-protein) [pubmed|31552824]
[PDB|6D9R] (complex with the substrate PRPP) [pubmed|31552824]
[PDB|6D9S] (complex with ppGpp) [pubmed|31552824]
[AF|P37472]
Effectors of protein activity
inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´, (p)ppGpp binds to the acive site of the enzyme and prevents the conversion of the apo-tetramer to the active dimer [Pubmed|31552824,22981860]
Kinetic information
KM (PRPP):  166 µM [pubmed|31552824]
KI (pppGpp): 1.7 µM [pubmed|31552824]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[Pubmed|18179421]
regulation
induced by heat shock (class III)
regulatory mechanism
[protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]: activation, with [protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT] [Pubmed|24001521], in [regulon|protein:473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS regulon]
[protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]: activation, with [protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS] [Pubmed|24001521], in [regulon|protein:B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7608085], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Open in new tab

[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]→[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]

2022-07-19 01:46:02

ghost

32

0b831aee45fd6cc4e3e06cbf53fe7330f12b4cb1

0B2B3D2B74921E6EEF5704482CF1D3DF309FA3D3

genes
[gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]-[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]-[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]-[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[pubmed|22383849]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|16497325]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Open in new tab

[gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]->[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]

2022-07-18 01:30:44

Jstuelk

28

7069328a463a18ae1e3c27f03ddf76f79efe7c06

8FBB4B976CF46DAD9437300BBE1B22B618D33ED0

Biological materials
Mutant
BKE00680 (Δ[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]::erm  trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATGATTTTGCTTGCCC,  downstream forward: _UP4_TGATCGGCAGCCTGCTTCCG
BKK00680 (Δ[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]::kan  trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATGATTTTGCTTGCCC,  downstream forward: _UP4_TGATCGGCAGCCTGCTTCCG
References
Reviews
28031352
Original Publications
18179421,22981860,24001521,6408059,28189581,31552824

B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738

Page visits: 3017

Time of last update: 2022-08-06 19:28:56

Author of last update: Jstuelk