rnc

rnc
168

RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria

locus
BSU_15930
Molecular weight
28.28 kDa
pI
8.08
Protein length
Gene length
function
processing and degradation of RNA molecules
product
endoribonuclease III
essential
yes
synonyms
rnc, rncS

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0571

This gene is a member of the following regulons

Gene
Coordinates
1,665,710 → 1,666,459
Phenotypes of a mutant
essential [Pubmed|23300471,12682299,11123676]
the ''[gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]'' gene can be deleted in strains cured of the [wiki|Skin element] and [wiki|SP-beta prophage] (or upon deletion of the toxin genes ''[gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]'' and ''[gene|961DB64225CEF84D80982ADBDC9BBBCDF5049132|yonT]'') [Pubmed|23300471]
additional information
[wiki|RNase III targets| RNAs that are targeted by RNase III] [pubmed|26883633]
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
cleaves 16S rRNA 2 nts downstream of the 3' end [PubMed|26883633]
cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]'')
degradation of ''[gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]'' mRNA-[protein|search|FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0] RNA duplex (encoded on the [wiki|Skin element]) [Pubmed|23300471]
degradation of ''[gene|961DB64225CEF84D80982ADBDC9BBBCDF5049132|yonT]'' mRNA-''[gene|8832F99BF927D5A4D6CB265DB24D3AE46434A5CD|SR6]'' RNA duplex (encoded on the [[SP-beta prophage]]) [Pubmed|23300471]
degradation of the ''[gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]''-''[gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]'' and ''[gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]-[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]'' RNA duplexes [Pubmed|25643072]
Protein family
ribonuclease III family (single member, according to UniProt)
[wiki|Domains]
RNase III domain (aa 20-149) (according to UniProt)
DRBM domain (aa 175-222) (according to UniProt)
Structure
[PDB|1O0W] (from ''Thermotoga maritima'', 41% identity)
[AF|P51833]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]-[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]-[gene|D519765BF5261E48512CE86245BD7B733F188D4A|ftsY]
description
[Pubmed|11021934]
regulation
expressed during vegetative growth and during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|11021934]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11021934], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]→[gene|D519765BF5261E48512CE86245BD7B733F188D4A|ftsY]

2023-02-01 13:38:17

ghost

51

6bc011f23705f0484f05e78a9ae631b907169aff

E7BDA851062351C16952CEF8C156BA6388FED18F

additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Stülke] lab, [pubmed|19193632]
labs
[wiki|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
[wiki|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
References
Reviews
17194582,16179796,8936814,16855311,23300472,25292357,25808661,25878039,29651979,31464530,35787606
Original Publications
9393702,11123676,7527387,9677377,17576666,11021934,6409421,22123973,22412379,25643072,23300471,26940229,26883633

BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981

Page visits: 2694

Time of last update: 2023-02-08 06:10:26

Author of last update: Jstuelk