rapJ

rapJ
168

response regulator aspartate phosphatase, dephosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]-P

locus
BSU_02820
Molecular weight
44.25 kDa
pI
4.9
Protein length
Gene length
function
control of the [wiki|phosphorelay]
product
response regulator aspartate phosphatase
essential
no
synonyms
rapJ, ycdE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0457

This gene is a member of the following regulons

Gene
Coordinates
304,430 → 305,551
The protein
Catalyzed reaction/ biological activity
dephosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]-P [Pubmed|21346797]
Protein family
[wiki|RAP family] (according to UniProt)
[wiki|Domains]
RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain (five [wiki|TPR repeat|tetratrichopeptide repeats]) that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide [protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC], the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. [Pubmed|23526881]
Structure
[PDB|4GYO] (complex [protein|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]-[protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]) [Pubmed|23526881]
[AF|O34327]
Effectors of protein activity
the interaction with [protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC] inactivates [protein|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ] [Pubmed|23526881]
Expression and Regulation
Operons
genes
[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]
description
[pubmed|22383849]
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[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]

2022-11-23 13:09:45

Jstuelk

27

2fed8fde2132bb7aa3b8371b27f7f996844785ad

8AB759F79AA0AD9C388B131287E3BA03B099AA75

Biological materials
Mutant
BKE02820 (Δ[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02820 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCTGCCTCCTTCCT,  downstream forward: _UP4_TAGGAAATGGCAGAGAACTA
BKK02820 (Δ[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02820 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCTGCCTCCTTCCT,  downstream forward: _UP4_TAGGAAATGGCAGAGAACTA
References
21346797,23526881

BC28167FE175BFD7DBF1B07C54D3512184B95B8F

Page visits: 1516

Time of last update: 2022-12-05 16:26:30

Author of last update: Melvin.boenninger