hutI
168
imidazolone-5-propionate hydrolase
locus
BSU_39370
Molecular weight
45.40 kDa
pI
5.22
function
histidine utilization
product
imidazolone-5-propionate hydrolase
essential
no
ec
3.5.2.7
synonyms
hutI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1228 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,045,245 → 4,046,510
The protein
Catalyzed reaction/ biological activity
4-imidazolone-5-propanoate + H2O --> N-formimidoyl-L-glutamate (according to UniProt)
Protein family
[wiki|Metallo-dependent hydrolases superfamily] (according to UniProt)
Structure
[PDB|2BB0] [Pubmed|16990261]
[AF|P42084]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP]-[gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH]-[gene|ED6002BB56B96F9A2B4F80CA050518B266000E55|hutU]-[gene|BD3382760B704972A0E67ECEDA4B29AA4ECD8164|hutI]-[gene|FBB15E07B7134C57208EA82D5339708385DBC5FB|hutG]-[gene|423AEC9D260BE53AD22851D999244B89BEB3E84D|hutM]
description
[Pubmed|10746760]
regulation
induced by histidine ([protein|search|HutP]) [Pubmed|8071225]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|8071225], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|8682780], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP]: antitermination, at a protein-dependent [wiki|RNA switch] located between [gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP] and [gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH] [Pubmed|8071225], in [regulon|protein:BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8071225], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE39370 (Δ[gene|BD3382760B704972A0E67ECEDA4B29AA4ECD8164|hutI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCTTTGGCATGTCATCAC, downstream forward: _UP4_GTTGTCAACAGGGAGGGAGC
BKK39370 (Δ[gene|BD3382760B704972A0E67ECEDA4B29AA4ECD8164|hutI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCTTTGGCATGTCATCAC, downstream forward: _UP4_GTTGTCAACAGGGAGGGAGC
References
Reviews
Original Publications
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Time of last update: 2025-07-22 03:33:28
Author of last update: Melvin.boenninger