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nrdF

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nrdF
168

ribonucleoside-diphosphate reductase (beta subunit)

locus
BSU_17390
Molecular weight
38.22 kDa
pI
4.66
Protein length
Gene length
function
synthesis of deoxyribonucleoside triphosphates
product
ribonucleoside-diphosphate reductase (beta subunit)
essential
yes
ec
1.17.4.1
synonyms
nrdF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0208

This gene is a member of the following regulons

Gene
Coordinates
1,871,089 → 1,872,078
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
[thioredoxin]-disulfide + 2'-deoxyribonucleoside 5'-diphosphate + H2O --> [thioredoxin]-dithiol + ribonucleoside 5'-diphosphate (according to UniProt)
required for stationary-phase mutagenesis [Pubmed|27920297]
Protein family
ribonucleoside diphosphate reductase small chain family (with [protein|08B733574DC7B8D044033EBB94124D470A0FBE19|yosP], according to UniProt)
[wiki|Cofactors]
dimanganic-tyrosyl radical (MnIII2-Y•) cofactor [Pubmed|24401092]
Structure
[PDB|4DR0] [Pubmed|22443445]
[PDB|6MW3] (inhibitory filament formed with [protein|3B68CE8E9A35997B45E437A316996D585E43B76B|nrdE]) [Pubmed|31201319]
Effectors of protein activity
dATP at concentrations up to ~10 M stimulate CDP and UDP reduction, while higher concentrations inhibit this activity, inhibition by dATP is enhanced by binding of dAMP to the N-terminal domain of [protein|3B68CE8E9A35997B45E437A316996D585E43B76B|nrdE] [Pubmed|31201319,29712847,24401092]
Paralogous protein(s)
[protein|08B733574DC7B8D044033EBB94124D470A0FBE19|yosP]
Expression and Regulation
Operons
genes
[gene|1EA0595C74359A36B9161CFD531CA894C5E66E25|nrdI]-[gene|3B68CE8E9A35997B45E437A316996D585E43B76B|nrdE]-[gene|CD2D14FE9C10544E7C350167EBDBD941B898051D|nrdF]-[gene|8C2A08A8395B72CCC9D03A8154602B8F3B77A1AA|ymaB]
description
[Pubmed|16885274]
regulation
expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|16885274]
regulatory mechanism
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|16885274], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
[protein|FD4E988BB73F6476CCEA4C521F343D6F8CBA457F|nrdR]: repression, (predicted) [Pubmed|15949864], in [regulon|protein:FD4E988BB73F6476CCEA4C521F343D6F8CBA457F|nrdR regulon]
[protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]: transcription repression, [Pubmed|27920297], in [regulon|protein:00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16885274], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|ymzA]' and '[protein|search|nrdI]' [PubMed|20525796]
Open in new tab

[gene|1EA0595C74359A36B9161CFD531CA894C5E66E25|nrdI]→[gene|8C2A08A8395B72CCC9D03A8154602B8F3B77A1AA|ymaB]

2022-01-25 14:54:34

ghost

13

9d160ac30c7745496a34c93be2b3a105e0e35c39

32952B230D636C27ED0B2D37BD2B3FCE31A938B1

genes
[gene|93FA602438546FD6201FAB5BD19DB5DEB5965E48|ymzA]-[gene|1EA0595C74359A36B9161CFD531CA894C5E66E25|nrdI]-[gene|3B68CE8E9A35997B45E437A316996D585E43B76B|nrdE]-[gene|CD2D14FE9C10544E7C350167EBDBD941B898051D|nrdF]-[gene|8C2A08A8395B72CCC9D03A8154602B8F3B77A1AA|ymaB]
description
[Pubmed|22383849]
Open in new tab

[gene|93FA602438546FD6201FAB5BD19DB5DEB5965E48|ymzA]→[gene|8C2A08A8395B72CCC9D03A8154602B8F3B77A1AA|ymaB]

2022-01-25 14:54:36

Jstuelk

16

c327eefb1356b60d6946dc8bb1110dce66ded764

3940914A8E31A6E8EACC40EBB2BDE0A966EB921D

Biological materials
Expression vectors
pGP2894: expression of Strep-''nrdF'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Elisabeth Härtig], Braunschweig, Germany [http://www.tu-braunschweig.de/ifm/abt/jahn/groups/bs Homepage]
References
Reviews
17229208,32569524
Original Publications
16885274,8969495,24401092,23402532,22443445,21561096,15949864,27920297,28624879,31201319,34031038

CD2D14FE9C10544E7C350167EBDBD941B898051D

Page visits: 1418

Time of last update: 2022-01-26 01:04:12

Author of last update: Robert.warneke