murG

murG
168

UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, conversion of lipid I to lipid II

locus
BSU_15220
Molecular weight
39.82 kDa
pI
9.57
Protein length
Gene length
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
essential
yes
ec
2.4.1.227
synonyms
murG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0707

This gene is a member of the following regulons

Gene
Coordinates
1,591,540 → 1,592,631
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
converts lipid I to lipid II by attaching GlcNAc to the bactoprenol carrier-conjugated UDP-N-acetylmuramic acid (UDP-MurNAc) pentapeptide molecule
Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol + UDP-N-acetyl-α-D-glucosamine --> β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol + H+ + UDP (according to UniProt)
Protein family
glycosyltransferase 28 family (with [protein|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP] and [protein|25CECB2D3C7925408DB96A156745BAD6A6FAB0AC|ykoN], according to UniProt)
Structure
[PDB|1F0K] (from ''Escherichia coli'', 30% identity, 52% similarity) [Pubmed|10892798]
[AF|P37585]
[wiki|Localization]
peripheral membrane protein [Pubmed|24706874]
in the presence of daptomycin, MurG delocalizes from the membrane to the cytoplasm [Pubmed|27791134]
forms banded patterns along the length of the cell, this pattern formation depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] [Pubmed|17880425]
Expression and Regulation
Operons
genes
[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
description
[Pubmed|18179421]
regulation
expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|1391053]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, (promoter within [protein|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]) [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8106328], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8320223], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Open in new tab

[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]→[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]

2022-04-27 17:36:36

Jstuelk

19

5a24cdfec598f0a2424898f47e06e5544408d3d6

1A7F525A9A274FAF9AE05A426F5AE7383E95D9E3

genes
[gene|D48B7591467C493D2214D37AEA83643FE8C27BC6|murE]-[gene|4D3D4429E1F387BACB0D5A1084AA8DF179A16685|mraY]-[gene|0A9FF5A66F73464A780914A1424979CE753B3D9C|murD]-[gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]-[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
description
[Pubmed|1391053]
regulation
expressed during vegatative growth [Pubmed|1391053]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8320223], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Open in new tab

[gene|D48B7591467C493D2214D37AEA83643FE8C27BC6|murE]→[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]

2022-04-27 17:36:38

Jstuelk

21

68c6899b3f1263bd636c35a4ea9512c00078a714

2F040145AC628A38E3D11955965F4DA256DE6790

genes
[gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]-[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
description
[Pubmed|1391053]
regulation
expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|1391053]
Open in new tab

[gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]→[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]

2022-06-13 13:26:40

Jstuelk

21

6fa043950e40c3a1d4f91339581472acb9db18da

C066C3357FB70D8EEB595C678C068B56731F8F59

Biological materials
FLAG-tag construct
GP2002 ''murG-3xFLAG ermC'' (based on [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab
References
22092867,18063720,18081839,1391053,8320223,15383836,18179421,10790316,24706874,26278972,17880425,20233306,27791134

D52FDB5015B10E9B26D7FED5708457890C6EA43D

Page visits: 4459

Time of last update: 2022-06-27 07:56:46

Author of last update: Melvin.boenninger