murAA

At Gram+ 2022 in Prague, there will be a workshop on how to get the most out of SubtiWiki. We will present newly added features. Moreover, we hope to learn from you what you would like to have added to SubtiWiki to improve it!

murAA
168

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

locus
BSU_36760
Molecular weight
46.54 kDa
pI
5.45
Protein length
Gene length
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
essential
yes
ec
2.5.1.7
synonyms
murAA, murA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0766

This gene is a member of the following regulons

Gene
Coordinates
3,777,949 → 3,779,259
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
phosphoenolpyruvate + UDP-N-acetyl-α-D-glucosamine --> phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-α-D-glucosamine (according to UniProt)
Protein family
EPSP synthase family (with [protein|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE] and [protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB], according to UniProt)
Structure
[PDB|3SG1] (from ''B. anthracis'', 79% identity, 94% similarity)
[AF|P70965]
Paralogous protein(s)
[protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
[protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA] is subject to [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-dependent degradation upon glucose starvation  [Pubmed|14763982,17981983]
Expression and Regulation
Operons
genes
[gene|32CE4F77F08F926152BECAB56E2841E1FFB27600|ywzB]-[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
description
[Pubmed|22383849]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22383849], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|26735940], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Open in new tab

[gene|32CE4F77F08F926152BECAB56E2841E1FFB27600|ywzB]→[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]

2022-05-06 12:10:44

Bzhu

13

348b92db57f702141368550bd6f8ee854f499e36

A3D075B597018E5E660B1BB16E5A4B840E277744

genes
[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
description
[Pubmed|9353933]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]
Open in new tab

[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]

2022-05-17 13:49:08

Bzhu

17

ab428b098c444d6051864b940c5bd49c48b8adce

E9A78A0A5F737601805178857AA7B293094805B4

genes
[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
description
[Pubmed|26735940]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|26735940]
Open in new tab

[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]→[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]

2022-05-17 08:36:00

Bzhu

15

8686823a2584a3b9acd44b2f60efbd7dabdbf1a7

EC335D8C98E1864C25DE56486949E2C94D39B0B9

additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Expression vectors
pRSETA-murAA, Expression of His(6)-MurAA, avalable in [wiki|Ulf Gerth]'s lab [Pubmed|14763982]
pGP2597: (IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
Antibody
avalable in [wiki|Ulf Gerth]'s lab [Pubmed|14763982]
References
Reviews
21822642
Original Publications
14763982,18063720,19899805,14763982,8994972,9485407,26735940,22383849,26883633

DA763F7C2192655C590752C2A285ADF902B11BCA

Page visits: 2871

Time of last update: 2022-05-18 20:27:19

Author of last update: Melvin.boenninger