walK

walK
168

two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with [wiki|cell division] by controlling the transcription of genes for autolysins and their inhibitors

locus
BSU_40400
Molecular weight
69.86 kDa
pI
4.78
Protein length
Gene length
function
control of cell wall metabolism
product
two-component sensor kinase
essential
yes
ec
2.7.13.3
synonyms
walK, yycG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5002

This gene is a member of the following regulons

Gene
Coordinates
4,151,853 → 4,153,688
Phenotypes of a mutant
essential [Pubmed|12682299]
essentiality can be bypassed by constitutive expression of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] or [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] in a [gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC] [gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] mutant background [pubmed|29465029]
a mutant (point mutation) has been selected during growth at low pressure [Pubmed|26296725]
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
Protein family
two-component sensor kinase
[wiki|Domains]
two transmembrane segments
[wiki|HAMP domain] (aa 204-256) (according to UniProt)
[wiki|PAS domain] (aa 263-324) (according to UniProt)
[wiki|PAC domain] (aa 325-379) (according to UniProt)
[wiki|Histidine kinase domain] (aa 383-602) (according to UniProt)
Structure
[PDB|3SL2] (complex of the ATP binding histidine kinase domain with ATP) [Pubmed|22751669]
[AF|Q45614]
Modification
autophosphorylation on a His residue
Effectors of protein activity
activity is modulated in response to changes in D,L-endopeptidase activity (via the extracellular [wiki|PAS domain]) [pubmed|31808740]
Paralogous protein(s)
[protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE], [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]
[wiki|Localization]
cell membrane [Pubmed|21219466]
part of the division septum in growing cells, interacts with the [wiki|divisome] [Pubmed|18573169]
Expression and Regulation
Operons
genes
[gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
description
[Pubmed|9829949]
regulation
''htrC'' transcript expressed during sporulation [Pubmed|9829949]
Open in new tab

[gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]→[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]

2022-09-29 18:35:13

ghost

42

4e891bb3dceb8eda2e5ad4f9ed646a611afbe349

32BB8D419E54714C8925B07FBBCF691251CA4145

Biological materials
Mutant
MGNA-B827 (yycG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1826 NBRP B. subtilis, Japan]
Expression vectors
pGP2636, constitutive expression in ''B. subtilis'' (based on [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
19019149,18792692,10094672,18245295,28886686,31976860
Original Publications
18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157,19342776,20057515,20167622,21219466,23632918,22751669,22526318,24490131,24900578,26296725,28674376,29465029,29465029,31339578,31808740

E88978809272FAC2520DB4ABCA0554F8028F3451

Page visits: 3807

Time of last update: 2022-10-06 17:55:07

Author of last update: Jstuelk