ycsA
168
tartrate dehydrogenase
locus
BSU_04000
Molecular weight
38.67 kDa
pI
5.11
function
utilization of tartrate
product
tartrate dehydrogenase
essential
no
ec
1.1.1.93
synonyms
ycsA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0473 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
452,830 → 453,894
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The protein
Catalyzed reaction/ biological activity
L-tartrate + NAD+ --> oxaloglycolate + NADH [pubmed|38127181]
Dihydroxyfumarate + NADH --> L-tartrate + NAD+ [pubmed|38127181]
meso-tartrate + NAD+ --> D-glycerate + CO2 + NADH [pubmed|38127181]
Protein family
Isocitrate and isopropylmalate dehydrogenases family (with [protein|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd] and [protein|105E3452D7F142A7D7616E35AF3FB753C9B63E38|leuB], according to UniProt)
[wiki|Cofactors]
Mn2+ [pubmed|38127181]
Structure
[PDB|3FMX] (from ''Pseudomonas Putida '', 51% identity)
[AF|P42958]
Paralogous protein(s)
[protein|105E3452D7F142A7D7616E35AF3FB753C9B63E38|leuB], [protein|CB7D0E32C044D541CD966DC1F9DA489D518A7703|icd]
Expression and Regulation
Operons
genes
[gene|E9D1B04FCB24402B56DC6A6F7AD696209E4BD0FA|ycsA]
description
[pubmed|22383849]
expression
expression is reduced upon over expression of [protein|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS] [pubmed|37338392]
regulatory mechanism
[protein|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]: sRNA, [pubmed|37338392], in [regulon|protein:6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS regulon]
Biological materials
Mutant
MGNA-C020 (ycsA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2018 NBRP B. subtilis, Japan]
BKE04000 (Δ[gene|E9D1B04FCB24402B56DC6A6F7AD696209E4BD0FA|ycsA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTTCCCCTGTTAT, downstream forward: _UP4_TGATGAATCAGGCCGGTGGC
BKK04000 (Δ[gene|E9D1B04FCB24402B56DC6A6F7AD696209E4BD0FA|ycsA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTTCCCCTGTTAT, downstream forward: _UP4_TGATGAATCAGGCCGGTGGC
References
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Time of last update: 2024-04-16 12:30:01
Author of last update: Jstuelk