gapA

gapA
168

glyceraldehyde 3-phosphate dehydrogenase, NAD-dependent, glycolytic enzyme, forms a transhydrogenation cycle with [protein|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB] for balancing of NADPH

locus
BSU_33940
Molecular weight
35.68 kDa
pI
5.03
Protein length
Gene length
function
catabolic enzyme in glycolysis
product
glyceraldehyde 3-phosphate dehydrogenase
essential
Yes
ec
1.2.1.12
synonyms
gapA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0057

This gene is a member of the following regulons

Gene
Coordinates
3,481,698 3,482,705
Phenotypes of a mutant
Essential [Pubmed|17114254]
The protein
Catalyzed reaction/ biological activity
D-glyceraldehyde 3-phosphate + NAD+ + phosphate --> 3-phospho-D-glyceroyl phosphate + H+ + NADH (according to UniProt)
This reaction is part of the glycolysis.
Protein family
glyceraldehyde-3-phosphate dehydrogenase family (with [protein|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB], according to UniProt)
[wiki|Cofactors]
NAD+ (does not accept NADP+) [Pubmed|10799476]
Structure
[PDB|1GD1] (from ''Geobacillus stearothermophilus'') [pubmed|3586018]
[PDB|1NQO] (from ''Geobacillus stearothermophilus'', mutant with cys 149 replaced by ser, complex with NAD and D-glyceraldehyde-3-phosphate) [pubmed|12569100]
[AF|P09124]
Modification
phosphorylated on Arg-199 [Pubmed|31221751,22517742]
Phosphorylation on (Ser-148 OR Ser-151 OR Thr-153 OR Thr-154) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed1], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed2]
Reversible thiol modifications after exposure to toxic quinones [Pubmed|18673455]
Cys152-Cys156 form intramolecular disulfide in response to disulfide stress (diamide, NaOCl-stress) [Pubmed|21749987]
Kinetic information
Michaelis-Menten [Pubmed|10799476]
Paralogous protein(s)
[protein|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB]
[wiki|Localization]
cytoplasm (Homogeneous) [Pubmed|16479537] [Pubmed|14600241]
loosely membrane associated [Pubmed|18763711]
Additional information
GAP dehydrogenases from different sources (incl. ''Geobacillus stearothermophilus'') were shown to cleave RNA ([Pubmed|12359717])
Moreover, mutations in ''gapA'' from ''B. subtilis'' can suppress mutations in genes involved in DNA replication ([Pubmed|17505547]).
extensive information on the structure and enzymatic properties of GapA can be found at [http://www.proteopedia.org/wiki/index.php/Glyceraldehyde-3-Phosphate_Dehydrogenase Proteopedia]
belongs to the 100 most abundant proteins [PubMed|15378759]
the protein is stable under both glycolytic and gluconeogenic conditions [pubmed|28760849]
Expression and Regulation
Operons
genes
[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
description
[Pubmed|11489127]
regulation
expression induced by glycolytic intermediates ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] [Pubmed|11489127]
the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ymcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT] complex [Pubmed|29794222]
regulatory mechanism
[protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [Pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]→[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]

2022-12-03 04:27:19

Jstuelk

53

7b5a04e0f51be88bb9a430514dd0cb9d39ea3a2c

24906FF9311EDEA4AFD94747BD67B2022E642FAB

genes
[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]
description
[Pubmed|11489127]
regulation
expression induced by glucose (10 fold) ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [Pubmed|12850135,12622823]
the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ymcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT] complex [Pubmed|29794222]
regulatory mechanism
[protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]→[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]

2022-11-24 19:12:02

Jstuelk

55

42c171759b28a8125169e493f230a3f3dfc6e9b8

312D000A710534F6E0BADE1077DFF609B6028623

Biological materials
Mutant
GP592 (''gapA''::''cat''), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
GP597 (''gapA''::''erm''), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
GP703 (''gapA''::''cat'' ''[gene|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB]''::''spec''), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
GM1501 (under p(spac) control), available in [wiki|Stephane Aymerich]'s lab
1A1003 ( ''gapA''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1003&Search=1A1003 BGSC]
Expression vectors
pGP1424 (expression in ''B. subtilis'', in [wiki|pBQ200]) (available in [wiki|Jörg Stülke]'s lab)
pGP90 (N-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP380]) (available in [wiki|Jörg Stülke]'s lab) [pubmed|19193632]
pGP704 (N-terminal His-tag, in [wiki|pWH844]) (available in [wiki|Jörg Stülke]'s lab) [pubmed|12634343]
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
lacZ fusion
pGP506 (in [wiki|pAC7]), pGP512 (in [wiki|pAC6]) (available in [wiki|Jörg Stülke]'s lab)
Antibody
available in [wiki|Jörg Stülke]'s lab [pubmed|12634343]
labs
[wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
[wiki|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html homepage]
References
Reviews
11163353,27750368,28271485,34066298
Original Publications
22517742,23420519,22740702,23034808,21815947,21749987,12850135,19193632,18673455,20444087,17726680,16479537,12622823,12359717,10799476,17505547,11489127,12123463,17218307,12634343,17142398,17114254,10559165,15378759,27449348,28760849,28818119,3586018,12569100,29794222,31221751

EB6512177418B1601C6641FB2DEE99C2CD10E671

Page visits: 7279

Time of last update: 2022-12-06 13:04:14

Author of last update: Jstuelk