glpT
168
[metabolite|glycerol-3-phosphate] permease
locus
BSU_02140
Molecular weight
49.64 kDa
pI
9.37
function
[metabolite|glycerol-3-phosphate] uptake
product
glycerol-3-phosphate permease
essential
no
synonyms
glpT, ybeE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2271 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
233,994 → 235,328
The protein
Catalyzed reaction/ biological activity
uptake of [metabolite|glycerol-3-phosphate]
Protein family
[wiki|major facilitator superfamily] (according to UniProt)
Structure
[PDB|1PW4] (from E. coli, 60% identity) [pubmed|12893936]
[AF|P37948]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|ECCBD8EF461C24D693450E10EC268DBC7A440618|glpT]-[gene|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ]
description
[Pubmed|8012593]
regulation
induced by glycerol ([protein|search|GlpP]) [Pubmed|8012593]
regulatory mechanism
[protein|38767691AE7E09F46B9E97A60BF5358C1876EDF8|glpP]: antitermination, via a protein-dependent [wiki|RNA switch] [Pubmed|8012593], in [regulon|protein:38767691AE7E09F46B9E97A60BF5358C1876EDF8|glpP regulon]
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|10913081], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8012593], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
increased expression at increased temperature (via an RNA thermometer that covers the Ribosomal binding site of the mRNA) [pubmed|37217261]
Biological materials
Mutant
QB5437 (ermC), available in the [wiki|Stülke] lab
BKE02140 (Δ[gene|ECCBD8EF461C24D693450E10EC268DBC7A440618|glpT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATAATTATCCCCCTT, downstream forward: _UP4_TAAGAAAGACACATAAAAGA
BKK02140 (Δ[gene|ECCBD8EF461C24D693450E10EC268DBC7A440618|glpT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATAATTATCCCCCTT, downstream forward: _UP4_TAAGAAAGACACATAAAAGA
References
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Time of last update: 2025-07-02 05:11:20
Author of last update: Jstuelk