ahpF

168

alkyl hydroperoxide reductase (large subunit) / NADH dehydrogenase

locus: BSU_40100

Molecular weight: 54.71 kDa

pI: 4.7

Protein length: 509 aa

Gene length: 1530 bp

function: resistance against peroxide stress

product: alkyl hydroperoxide reductase (large subunit) / NADH dehydrogenase

essential: no

ec: 1.6.99.3

synonyms: ahpF, ndh

List of homologs in different organisms, belongs to COG3634 (Galperin et al., 2021)

Gene

Coordinates: 4,119,527  4,121,056

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The protein

Catalyzed reaction/ biological activity

• A + H⁺ + NADH --> AH₂ + NAD⁺ (according to UniProt)

Protein family

• class-II pyridine nucleotide-disulfide oxidoreductase family (with [protein|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB], according to UniProt)

[wiki|Domains]

• Thioredoxin-like fold domain (aa 108-210) (according to InterPro)

[wiki|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|1HYU] (from ''Salmonella typhimurium'', 52% identity, 69% similarity) [Pubmed|11300769]
• [AF|P42974]

Modification

• phosphorylation on (Ser-48 OR Ser-49) [Pubmed|17218307]
• phosphorylated on Arg-457 [Pubmed|22517742]

[wiki|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|D0982500E52577D52FADF775C0E512A4B9657B79|ahpC]-[gene|8F23B575BC32EA63267C4321DD8E4BD546B11AF1|ahpF]

  description: [Pubmed|8932314]

  regulation

  • induced by H2O2 ([protein|search|PerR]) [Pubmed|11532148]

  regulatory mechanism

  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]: repression, [Pubmed|11532148], in [regulon|protein:00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8932314], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• GP1730 [gene|search|ahpCF]::mls trpC2 available in [wiki|Jörg Stülke]'s lab
• BKE40100 ([gene|8F23B575BC32EA63267C4321DD8E4BD546B11AF1|ahpF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCAAGTACCAATTGAATGC,  downstream forward: _UP4_TAATATAAGAAATCCGCTAT
• BKK40100 ([gene|8F23B575BC32EA63267C4321DD8E4BD546B11AF1|ahpF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCAAGTACCAATTGAATGC,  downstream forward: _UP4_TAATATAAGAAATCCGCTAT

References

• 11532148,16232966,8932314,8932315,11532148,17218307,22517742,15581580