alaT

168

PLP-dependent L-alanine aminotransferase

locus: BSU_31400

Molecular weight: 42.15 kDa

pI: 5.11

Protein length: 386 aa

Gene length: 1161 bp

function: biosynthesis of L-alanine

product: PLP-dependent L-alanine aminotransferase

essential: no

synonyms: alaT, yugH

List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)

Gene

Coordinates: 3,225,772  3,226,932

Phenotypes of a mutant

• strongly impaired growth in the absence of alanine [pubmed|33155333]
• a [gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT] [gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat] double mutant is auxotrophic for alanine [pubmed|33155333]

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The protein

Catalyzed reaction/ biological activity

• pyruvate + glutamate --> L-alanine + 2-oxoglutarate [pubmed|33155333]

Protein family

• [wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

Structure

• [PDB|1DJU] (from ''Pyrococcus horikoshii'', 47% identity) [Pubmed|10671523]
• [AF|Q795M6]

Paralogous protein(s)

• [protein|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB], [protein|094AE3AC8DFAFC8048ADAAB34F76DF2562D80CE7|patA]

Expression and Regulation

Operons

• genes: [gene|1CA87275AFA1B4E4B5E8E10229009F785E70BBA8|alaR]-[gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]

  description: [Pubmed|22383849]

  additional information

  • A [protein|search|ncRNA] is predicted between [gene|B0908BB4B8B569A7A78BB0B84784FD4C427C598B|yugI] and [gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT] [PubMed|20525796]

Biological materials

Mutant

• GP3594 (Δ[gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::''phleo''), available in [wiki|Jörg Stülke]'s lab
• GP3575 (Δ[gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::''kan''), available in [wiki|Jörg Stülke]'s lab
• MGNA-A621 (yugH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/621 NBRP B. subtilis, Japan]
• BKE31400 ([gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31400 BGSC] and in [wiki|Jörg Stülke]'s lab,  [Pubmed|28189581], upstream reverse: _UP1_ATAGTCTGATAAATACGAAG,  downstream forward: _UP4_TAAAAAAAGATACGAACCTT
• BKK31400 ([gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATAGTCTGATAAATACGAAG,  downstream forward: _UP4_TAAAAAAAGATACGAACCTT

References

• 20525796,22383849,10671523,33155333