melD

168

[wiki|ABC transporter] subunit for melibiose and raffinose (permease)

locus: BSU_30280

Molecular weight: 34.01 kDa

pI: 9.08

Protein length: 303 aa

Gene length: 912 bp

function: uptake of melibiose and raffinose

product: [wiki|ABC transporter] (permease)

essential: no

synonyms: melD, amyD

List of homologs in different organisms, belongs to COG1175 (Galperin et al., 2021)

Gene

Coordinates: 3,099,123  3,100,034

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The protein

Protein family

• [wiki|Binding-protein-dependent transport system permease family] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transmembrane type-1 domain] (aa 79-291) (according to UniProt)

Structure

• [PDB|4TQU] (from Sphingomonas sp., 30% identity) [pubmed|26235029]
• [AF|O34706]

[wiki|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]

  description: [Pubmed|22383849]

  regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]

• genes: [gene|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]-[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]

  description: [Pubmed|22383849]

  regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]: repression, [pubmed|31138628], in [regulon|protein:58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22900538,31138628], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A799 (amyD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/799 NBRP B. subtilis, Japan]
• BKE30280 ([gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCACATGAACGTCCCTTG,  downstream forward: _UP4_AAAAGAAAGGAGACGGAAAT
• BKK30280 ([gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCACATGAACGTCCCTTG,  downstream forward: _UP4_AAAAGAAAGGAGACGGAAAT

References

• 10092453,22383849,22900538,26235029,31138628