atpA

168

ATP synthase, part of the F1 complex (subunit alpha)

locus: BSU_36830

Molecular weight: 54.43 kDa

pI: 5.04

Protein length: 502 aa

Gene length: 1509 bp

function: ATP synthesis

product: ATP synthase (subunit alpha)

essential: no

ec: 3.6.3.14

synonyms: atpA

List of homologs in different organisms, belongs to COG0056 (Galperin et al., 2021)

Gene

Coordinates: 3,783,878 3,785,386

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The protein

Catalyzed reaction/ biological activity

• ATP + 4 H⁺ + H₂O --> ADP + 5 H⁺ + phosphate (according to UniProt) [http://www.youtube.com/watch?v=uOoHKCMAUMc see a video]

Protein family

• ATPase alpha/beta chains family (with [protein|401459F105BC8A8342341D953AE259D6C3868AB6|fliI] and [protein|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD], according to UniProt)

Structure

• [PDB|1SKY] ([protein|E3FADE979CE6AB6315F67812608FCE00CF4E2453|atpA]-[protein|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD] complex from ''Bacillus sp.'', 88% identity) [Pubmed|9261073]
• [PDB|see here an overview on ATPase structure]
• [AF|P37808]

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

Effectors of protein activity

• ATPase activity is inhibited upon binding of Mg-ADP to [protein|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD] [pubmed|30580998]

[wiki|Localization]

• membrane [Pubmed|18763711]
• peripheral via theF0 complex

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|8027AD5C3A92FB9B70E42C119FDEC697A64618C4|atpI]-[gene|CC1894D90AC17487A286B2909964916825055F93|atpB]-[gene|6CF7F6303425D8A51E1E01BF18823139E4259A8F|atpE]-[gene|87311FEA265AD3F5526C78D631E7E51BBF01F0D6|atpF]-[gene|E956A30D97B2941108880F3AD48620C4DB6E0EBD|atpH]-[gene|E3FADE979CE6AB6315F67812608FCE00CF4E2453|atpA]-[gene|492EEC8068043DA770F5DC1D54799C4366AE9A01|atpG]-[gene|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD]-[gene|A9DA64F8C7A60E8E2B1EDC99BEA1BD7DD0135AA6|atpC]

  description: [Pubmed|9353933]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the mRNA is processed between [gene|8027AD5C3A92FB9B70E42C119FDEC697A64618C4|atpI] and [gene|CC1894D90AC17487A286B2909964916825055F93|atpB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

Biological materials

Mutant

• BKE36830 ([gene|E3FADE979CE6AB6315F67812608FCE00CF4E2453|atpA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCTCACTTAGGTTTCAC, downstream forward: _UP4_TAACTCGAATGCTGATGAGA
• BKK36830 ([gene|E3FADE979CE6AB6315F67812608FCE00CF4E2453|atpA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCTCACTTAGGTTTCAC, downstream forward: _UP4_TAACTCGAATGCTGATGAGA

References

Reviews

• 23356252,23341301,23267178,22822068,21524994,19489730,17208001,16730323

Research papers

• 30580998

Original Publications

• 36815589,7961438,15378759,16493705,18763711,25244289,9261073,26883633