bcaP

168

amino acid transporter, major transporter for isoleucine, valine and threonine, minor serine and asparagine transporter

locus: BSU_09460

Molecular weight: 49.55 kDa

pI: 9.73

Protein length: 465 aa

Gene length: 1398 bp

function: uptake of branched-chain amino acids, threonine, serine, and asparagine

product: amino acid transporter

essential: no

synonyms: bcaP, yhdG

List of homologs in different organisms, belongs to COG0531 (Galperin et al., 2021)

Gene

Coordinates: 1,023,350  1,024,747

Phenotypes of a mutant

• no phenotype for the single mutant, the triple ''[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP] [gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ] [gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations [Pubmed|25645558]
• resistant to growth inhibition by high concentrations of threonine [Pubmed|32743959,25645558]
• resistant to 4-hydroxythreonine [pubmed|25777134]

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The protein

Catalyzed reaction/ biological activity

• high affinity uptake of isoleucine and valine [Pubmed|25645558], (symport with H⁺)
• uptake of threonine [pubmed|32743959], (symport with H⁺)
• uptake of serine [pubmed|32743959], (symport with H⁺)
• uptake of asparagine [pubmed|38193659], (symport with H⁺)
• uptake of diaminopropionic acid [pubmed|38470260], (symport with H⁺)

Protein family

• [wiki|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

Structure

• [PDB|5OQT] (from Geobacillus kaustophilus, 60% identity) [pubmed|29416041]
• [AF|O07576]

Paralogous protein(s)

• [protein|7E180420EB053D9EF7993EF600A169C446F905F9|mtrA]

[wiki|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]

  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]

Biological materials

Mutant

• GP4157 (Δ[gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]::''aphA3'', Δ[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::''ermC''), available in [wiki|Jörg Stülke]'s lab
• GP4484 (Δ[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::''ermC''), available in [wiki|Jörg Stülke]'s lab [pubmed|38470260]
• MGNA-B479 ([gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::''erm''), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1478 NBRP B. subtilis, Japan]
• BKE09460 ([gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09460 BGSC] and in [wiki|Fabian Commichau]'s and [wiki|Jörg Stülke]'s labs),  [Pubmed|28189581], upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA,  downstream forward: _UP4_TAACCTTTTGATAAAGAGAG
• BKK09460 ([gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA,  downstream forward: _UP4_TAACCTTTTGATAAAGAGAG

References

• 18083814,12618455,20935095,18763711,21097623,21699902,25645558,25777134,29416041,32743959,38193659,38470260