comFC

168

late competence gene, resembles type 1 phosphoribosyl transferases

locus: BSU_35450

Molecular weight: 26.26 kDa

pI: 8.85

Protein length: 229 aa

Gene length: 690 bp

function: nucleotide scavenging

product: unknown

essential: no

synonyms: comFC

List of homologs in different organisms, belongs to COG1040 (Galperin et al., 2021)

Gene

Coordinates: 3,641,125  3,641,814

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The protein

Protein family

• comF/gntX family (single member, according to UniProt)

Structure

• [AF|P39147]

Modification

• phosphorylated on Arg-74 [Pubmed|22517742]

[wiki|Localization]

• localizes to one or both cell poles [Pubmed|21278288]

Expression and Regulation

Operons

• genes: [gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|flgN]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]-[gene|E5A59D2AF53FA220BA98D9653589A8F0D24F75B8|yviE]-[gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]

  description: [Pubmed|22383849]

  expression

  • expression is heterogeneous [pubmed|35171018]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|8412657], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, [Pubmed|21736639], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|19898538], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8412657], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8045879], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]

Biological materials

Mutant

• BKE35450 ([gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGCGAATCACATAATAAAC,  downstream forward: _UP4_TAAATGATTCTGTTTTTATG
• BKK35450 ([gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGCGAATCACATAATAAAC,  downstream forward: _UP4_TAAATGATTCTGTTTTTATG

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

References

• 11814663,8412657,21278288,22517742,28618091,30254116,33527432,36815589