csbB

168

lipoteichoic acid glycosyltransferase, generation of C₅₅-P-Glc, general stress protein, required for protection against paraquate stress

locus: BSU_08600

Molecular weight: 37.57 kDa

pI: 9.2

Protein length: 329 aa

Gene length: 990 bp

function: lipoteichoic acid glycosylation , survival of stress conditions

product: lipoteichoic acid glycosyltransferase

synonyms: csbB, yfhN

List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

Gene

Coordinates: 930,818  931,807

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The protein

Protein family

• [wiki|glycosyltransferase 2 family] (according to UniProt)

Structure

• [PDB|5EKP] (from Synechocystis sp., 44% identity) [pubmed|26729507]
• [AF|Q45539]

Paralogous protein(s)

• [protein|EF18470AD2B07755431534B52A8FCE6754FDBA9D|ykoT], [protein|FD76A781162795EAF174B59C0A90D2ED3ACC0AB7|ykcC]

[wiki|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]-[gene|BDFB6CCB5AAD9747971DE6B78E07558706E04617|yfhO]

  description: [Pubmed|22383849,8921856]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|8921856,15805528,9636707]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|8921856,9636707], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|9636707], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]

Biological materials

Mutant

• MGNA-C319 (csbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2317 NBRP B. subtilis, Japan]
• BKE08600 ([gene|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAAGGCACCTTCTTTTT,  downstream forward: _UP4_TGAAAATACCAAGCGCCATT
• BKK08600 ([gene|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAAGGCACCTTCTTTTT,  downstream forward: _UP4_TGAAAATACCAAGCGCCATT

References

• 8921856,9636707,23632331,15805528,22582280,27185829,26729507,29343515