cspD

168

cold shock protein

locus: BSU_21930

Molecular weight: 7.17 kDa

pI: 4.31

Protein length: 66 aa

Gene length: 201 bp

function: RNA chaperone

product: cold shock protein

essential: no

synonyms: cspD

List of homologs in different organisms, belongs to COG1278 (Galperin et al., 2021)

Gene

Coordinates: 2,307,905 2,308,105

Phenotypes of a mutant

• [gene|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|cspB] [gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD] double mutants are poorly viable, rapidly acquire suppressor mutations that result in overexpression of [gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC] or loss of the [gene|B8BEE21D8E14F4B04B004573597EA26F5E6DAA39|veg] gene [pubmed|34361870]
• [gene|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|cspB] [gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD] double mutants have lost genetic competence [pubmed|34361870]

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The protein

[wiki|Domains]

• [wiki|CSD domain] (aa 4-63) (according to UniProt)

Structure

• [PDB|1C9O] (from ''B. caldolyticus'', 86% identity) [Pubmed|10736231]
• [AF|P51777]

Paralogous protein(s)

• [protein|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|cspB], [protein|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
• belongs to the 100 most abundant proteins [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD]

  description: [pubmed|22383849]

  expression

  • strong constitutive expression [Pubmed|34361870]

Biological materials

Mutant

• GP2614 ([gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD]::''aphA3''), available in [wiki|Jörg Stülke]'s lab [pubmed|34361870]
• MGNA-A874 (cspD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/874 NBRP B. subtilis, Japan]
• BKE21930 ([gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTAATTCCTCCT, downstream forward: _UP4_TAAACTCAATACATGATGAT
• BKK21930 ([gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTAATTCCTCCT, downstream forward: _UP4_TAAACTCAATACATGATGAT

Expression vectors

• pGP2164: expression of ''cspD''-Strep by [wiki|pGP382] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
• pGP2165: expression of Strep-''cspD'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [wiki|Jörg Stülke]'s lab

lacZ fusion

• GP3286 ([gene|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD]::lacZ cat), available in [wiki|Jörg Stülke]'s lab [pubmed|30957856]

References

Reviews

• 12171653,12838604

Original Publications

• 12399512,11752341,11717297,9920884,12427936,11591689,9379903,17981983,15378759,10736231,31876031,34157109,34361870