ctaO

168

heme O synthase (minor enzyme)

locus: BSU_12080

Molecular weight: 36.73 kDa

pI: 10.12

Protein length: 329 aa

Gene length: 990 bp

function: heme biosynthesis

product: heme O synthase (minor enzyme)

essential: no

ec: null

synonyms: ctaO, yjdK

List of homologs in different organisms, belongs to COG0109 (Galperin et al., 2021)

Gene

Coordinates: 1,279,514  1,280,503

Advertisement

The protein

Catalyzed reaction/ biological activity

• (2E,6E)-farnesyl diphosphate + H₂O + heme b --> diphosphate + Fe(II)-heme o (according to UniProt)

Protein family

• UbiA prenyltransferase family (with [protein|83F5D5FCCAFADB7231ACD9BE7941820240954C70|ctaB], according to UniProt)

Structure

• [AF|O31652]

Paralogous protein(s)

• [protein|83F5D5FCCAFADB7231ACD9BE7941820240954C70|ctaB]

[wiki|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|6CE139563BBD7C1C97AEC3AA3F88146C4EEA79E4|ctaO]

  description: [Pubmed|21815947]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

  additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-A273 (yjdK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/273 NBRP B. subtilis, Japan]
• BKE12080 ([gene|6CE139563BBD7C1C97AEC3AA3F88146C4EEA79E4|ctaO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTTTTGTTATCGTAAC,  downstream forward: _UP4_TGATGCAAAAAGAGCCACAC
• BKK12080 ([gene|6CE139563BBD7C1C97AEC3AA3F88146C4EEA79E4|ctaO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTTTTGTTATCGTAAC,  downstream forward: _UP4_TGATGCAAAAAGAGCCACAC

References

• 10675592,20817675,21815947