cwlC

168

N-acetylmuramoyl-L-alanine amidase

locus: BSU_17410

Molecular weight: 27.01 kDa

pI: 5.94

Protein length: 255 aa

Gene length: 768 bp

function: mother cell lysis

product: N-acetylmuramoyl-L-alanine amidase

essential: no

ec: 3.5.1.28

synonyms: cwlC

List of homologs in different organisms, belongs to COG3087 (Galperin et al., 2021)

Gene

Coordinates: 1,872,812  1,873,579

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The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• [wiki|N-acetylmuramoyl-L-alanine amidase 3 family] (according to UniProt)

[wiki|Domains]

• contains an amidase_3 domain (like [protein|B09FB626274B81F00A4FB42D188E089095343182|cwlD], [protein|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC], [protein|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|yqiI], [protein|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|yrvJ])
• [wiki|MurNAc-LAA domain] (aa 4-172) (according to UniProt)
• SPOR domain (aa 180-254) (according to UniProt)

Structure

• [PDB|1X60] (peptidoglycan binding domain) [pubmed|16042392]
• [AF|Q06320]

[wiki|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|cwlC]

  description: [Pubmed|8407798]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|8407798]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|8407798], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]

Biological materials

Mutant

• BKE17410 ([gene|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|cwlC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCATCACCTCATTA,  downstream forward: _UP4_TAGCCGAGACGGGGACGAGC
• BKK17410 ([gene|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|cwlC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCATCACCTCATTA,  downstream forward: _UP4_TAGCCGAGACGGGGACGAGC

References

• 10945275,16042392,11375403,8407798,35638784