cwlO

168

D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation

locus: BSU_34800

Molecular weight: 50.87 kDa

pI: 5.33

Protein length: 473 aa

Gene length: 1422 bp

function: cell wall synthesis, cell elongation

product: endopeptidase-type autolysin

essential: no

synonyms: cwlO, yzkA, yvcE

List of homologs in different organisms, belongs to COG3883 (Galperin et al., 2021)

Gene

Coordinates: 3,574,363  3,575,784

Phenotypes of a mutant

• a [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] mutant is not viable [Pubmed|17581128,22139507]
• a [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] [gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] mutant is not viable, due to lack of expression of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] [Pubmed|36265902]
• a [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] [gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP] mutant is not viable, due to lack of processing of [protein|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI] and the resulting inactivity of [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] [Pubmed|36265902]
• a [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] [gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA] mutant is not viable, due to lack of processing of [protein|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI] and the resulting inactivity of [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] [Pubmed|36265902]
• a [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] [gene|665BE3614B466067D64D1FB275ECDDE12CBFC9D4|ecsB] mutant is not viable, due to lack of processing of [protein|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI] and the resulting inactivity of [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] [Pubmed|36265902]
• shorter, fatter cells, this can be rescued by addition of Mg(2+) [Pubmed|23869552,23855774]
• the mutant cells are thicker and shorter than wild type cells [pubmed|34846166]
• loss of genetic competence [pubmed|29553055]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]
• improved survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]

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The protein

Catalyzed reaction/ biological activity

• cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]
• degradation of gamma-polyglutamic acid [pubmed|29458655]

Protein family

• [wiki|Peptidase C40 family] (according to UniProt)

[wiki|Domains]

• C-terminal D,L-endopeptidase domain ([wiki|NlpC/P60 domain]) [pubmed|29458655,22139507]

Structure

• [AF|P40767]

Effectors of protein activity

• [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] requires activation by [protein|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|ftsE]-[protein|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|ftsX] [Pubmed|23869552,23855774]
• activity requires functional [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [Pubmed|23869552]
• both enzymatic activities are inhibited by interaction with [protein|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] [pubmed|29458655]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]
• localizes to the outer lateral sidewall of the cell (via the N-terminal domain) [Pubmed|22139507]
• cell membrane in a [protein|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|ftsX]-dependent manner [Pubmed|23869552]

Biological materials

Mutant

• GP2642 ([gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO]::''aphA3''), available in [wiki|Jörg Stülke]'s lab
• MGNA-B643 (yvcE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1642 NBRP B. subtilis, Japan]
• BKE34800 ([gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTATATCCTCCCTT,  downstream forward: _UP4_TAATAAATATGACAAGGGCC
• BKK34800 ([gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTATATCCTCCCTT,  downstream forward: _UP4_TAATAAATATGACAAGGGCC

References

Reviews

• 23066944,18266855,33450852,33709487

Original Publications

• 21478646,16233686,17581128,20525796,18957862,20059685,22139507,23855774,23869552,24163346,27118079,29553055,29794222,29458655,29458657,29465029,34846166,34871030,35638827,36265902,36574412,37333659,37768080