dctS

168

two-component sensor kinase, regulation of the [gene|9A96572B101358768EB20EE9438BE0E1424A4130|dctS]-[gene|97C34F6FA0B89D93369150E9A8AB3579332B07BC|dctR]-[gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP] operon

locus: BSU_04450

Molecular weight: 59.77 kDa

pI: 6.2

Protein length: 535 aa

Gene length: 1608 bp

function: regulation of the [gene|9A96572B101358768EB20EE9438BE0E1424A4130|dctS]-[gene|97C34F6FA0B89D93369150E9A8AB3579332B07BC|dctR]-[gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP] operon

product: two-component sensor kinase

essential: no

synonyms: dctS, ydbF

List of homologs in different organisms, belongs to COG3290 (Galperin et al., 2021)

Gene

Coordinates: 497,768  499,375

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The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|97C34F6FA0B89D93369150E9A8AB3579332B07BC|dctR]
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

[wiki|Domains]

• two transmembrane segments
• [wiki|PAS domain] (aa 213–276) (according to UniProt)
• [wiki|Histidine kinase domain] (aa 333-528) (according to UniProt)

Structure

• [PDB|4I5S] (from Streptococcus mutans, corresponds to aa 199 ... 529, 22% identity) [pubmed|23468592]
• [AF|P96601]

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• [protein|7428E80AC67A659B8602AF887EA7DFFD4976B40C|dctB] and [protein|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP] act as co-sensors with [protein|9A96572B101358768EB20EE9438BE0E1424A4130|dctS] [Pubmed|24375102]

[wiki|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|9A96572B101358768EB20EE9438BE0E1424A4130|dctS]-[gene|97C34F6FA0B89D93369150E9A8AB3579332B07BC|dctR]-[gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]

  description: [Pubmed|10708364]

  regulation

  • part of the iron sparing response, strong down-regulation in a'' [protein|search|fur]'' mutant ([protein|search|Fur], [protein|search|FsrA]) [Pubmed|22389480]

Biological materials

Mutant

• GP225 (''dctS''::''cat''), available in [wiki|Jörg Stülke]'s lab
• BKE04450 ([gene|9A96572B101358768EB20EE9438BE0E1424A4130|dctS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGCTTCTCCTTTCAGG,  downstream forward: _UP4_CCAATGAAGGGGGAGGAAGC
• BKK04450 ([gene|9A96572B101358768EB20EE9438BE0E1424A4130|dctS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGCTTCTCCTTTCAGG,  downstream forward: _UP4_CCAATGAAGGGGGAGGAAGC

References

• 10094672,10708364,24375102,23468592