dhbB

dhbB
168

isochorismatase

locus
BSU_31970
Molecular weight
34.95 kDa
pI
4.43
Protein length
Gene length
function
biosynthesis of the siderophore bacillibactin
product
isochorismatase
essential
no
ec
3.3.2.1
synonyms
dhbB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3433 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,287,675  3,288,613
Phenotypes of a mutant
defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium [pubmed|31420537]
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The protein
Catalyzed reaction/ biological activity
Isochorismate + H2O --> 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate (according to UniProt)
Protein family
[wiki|Isochorismatase family] (according to UniProt)
[wiki|Domains]
[wiki|Carrier domain] (aa 229-302) (according to UniProt)
Structure
[PDB|2FQ1] (from E. coli, 49% identity) [pubmed|16632253]
[AF|P45743]
Expression and Regulation
Operons
genes
[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]-[gene|DD647083C2E6968AD26A41C9F4EFBA942D75207C|ybdZ]
description
[Pubmed|8921902]
regulation
induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,12354229]
expression is increased in the presence of pulchimerrin [pubmed|37137890]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]: repression, in [regulon|protein:65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8550523], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [pubmed|29914988], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
additional information
the ''[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]'' operon is strongly unregulated in a ''[wiki|kre]'' mutant [Pubmed|26110430]
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[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]→[gene|DD647083C2E6968AD26A41C9F4EFBA942D75207C|ybdZ]

2024-03-21 18:56:56

Jstuelk

80

875CBC1C026244834E2EFBC1140499985C0AB89D

CCA159D50C58E2078C51F543EDA686D77F6612EE

Biological materials
Mutant
BKE31970 ([gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGAACACTTCCTCTCA,  downstream forward: _UP4_TTATAAAAGGAGGGTAAACA
BKK31970 ([gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGAACACTTCCTCTCA,  downstream forward: _UP4_TTATAAAAGGAGGGTAAACA
References
11112781,8921902,11790741,12354229,8550523,20817675,23033921,21815947,26110430,29133393,29914988,16632253,31420537,35171018

1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA

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Time of last update: 2024-03-28 03:55:49

Author of last update: Jstuelk