dprA

dprA
168

conveys incoming ssDNA to [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]; facilitates the displacement of both SSBs ([protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]), increases [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex, crucial for the [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-dependent acquisition of homologous genes from related species by natural transformation

locus
BSU_16110
Molecular weight
32.78 kDa
pI
9.76
Protein length
Gene length
function
[category|SW.3.1.5|DNA repair/ recombination]
product
recombination mediator protein
essential
no
synonyms
dprA, smf

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0758 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,682,580 → 1,683,473
Phenotypes of a mutant
strongly impaired genetic recombination [Pubmed|23779106]
''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
loss of plasmid transformation [pubmed|30050509]
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The protein
Catalyzed reaction/ biological activity
binds ssDNA, promotes displacement of [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] and [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] from ssDNA [Pubmed|23779106]
provides [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] access to ssDNA during chromosomal transformation (together with [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]) [Pubmed|22373918]
[protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] as an accessory factor [Pubmed|25138221]
the [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] mediator loads [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] onto any fragmented linear SPP1 ssDNA [pubmed|31876108]
anneals [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]- or [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]-coated complementary strands of transfecting SPP1 phage DNA, yielding tailed SPP1 duplex intermediates [pubmed|31876108]
[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA], [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] or viral single strand annealing G35P protein, may catalyze the annealing of complete linear phage genomes with redundant regions at the ends of the molecule, alone or with the help of an exonuclease, to produce a circular unit-length duplex viral genome ready to initiate replication [pubmed|31876108]
Protein family
DprA/Smf family (single member, according to UniProt)
Structure
[PDB|3UQZ] (aa 90 ... 310, from Streptococcus pneumoniae, 44% identity) [pubmed|22904190]
[AF|P39813]
[wiki|Localization]
transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery [Pubmed|17630974]
Expression and Regulation
Operons
genes
[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]-[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]-[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
description
[Pubmed|22383849]
regulation
repressed by glucose (2.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|12850135]
expression is heterogeneous [pubmed|29809139]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
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[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]→[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]

2024-03-04 15:14:36

Jstuelk

51

167fd8fae94fab138af8eabe6101bc58adfb0921

2BC79D48E345B722DDAE396E448CF1DB3AAB049C

additional information
expressed under conditions that trigger genetic competence ([protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]) [Pubmed|11948146]
Biological materials
Mutant
BKE16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT,  downstream forward: _UP4_TGAATTATCGTTTGACAAAC
BKK16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16110 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT,  downstream forward: _UP4_TGAATTATCGTTTGACAAAC
labs
[wiki|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
References
Reviews
31950915
Original Publications
17630974,17803906,12421306,18045469,11948146,22373918,23779106,23440217,25138221,26786319,28618091,22904190,28911099,30050509,31876108,33264457

FC53216F600994C862F0C0D017C3FA28EB75DCB6

Page visits: 4805

Time of last update: 2024-03-28 23:36:48

Author of last update: Jstuelk