epsE

168

inhibitor of motility and glycosyltransferase required for EPS biosynthesis

locus: BSU_34330

Molecular weight: 32.05 kDa

pI: 9.8

Protein length: 278 aa

Gene length: 837 bp

function: biofilm formation

product: glycosyltransferase, inhibitor of motility

essential: no

synonyms: epsE, yveO

List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

Gene

Coordinates: 3,524,417  3,525,253

Phenotypes of a mutant

• smooth colonies on MsGG medium, no [wiki|biofilm formation] [Pubmed|22113911]

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The protein

Catalyzed reaction/ biological activity

• arrests flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-generating elements in cells embedded in the biofilm matrix, separates the cytoplasmic [protein|8DDCC3D139ACCB635BF014946E1282A72D535390|fliG] motor from the [protein|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]-[protein|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB] stator [Pubmed|18566286]
• biosynthesis of extracellular polysaccharides [Pubmed|21170308]

Protein family

• [wiki|glycosyltransferase 2 family] (according to UniProt)

Structure

• [AF|P71054]

Modification

• phosphorylated by [protein|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB] on a Tyr residue [Pubmed|25085422]

[wiki|Localization]

• cell membrane, forms spots at flagellar basal bodies [Pubmed|18566286]

Biological materials

Mutant

• MGNA-B613 (yveO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1612 NBRP B. subtilis, Japan]
• BKE34330 ([gene|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTCATACGCTTTTCTCCTT,  downstream forward: _UP4_AAGCATGAATAGCAGCCAAA
• BKK34330 ([gene|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTCATACGCTTTTCTCCTT,  downstream forward: _UP4_AAGCATGAATAGCAGCCAAA

labs

• [wiki|Daniel Kearns], Indiana University, Bloomington, USA, [http://www.bio.indiana.edu/facultyresearch/faculty/Kearns.html homepage]
• [wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

Reviews

• 20735481,24988880,25251856,31136265

Research papers

• 18566286,25085422,21170308,22113911

The EAR [wiki|RNA switch]

• 20374491,20230605