gerKD

168

negative effector (D protein) of the [protein|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[protein|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]-[protein|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC] germinant receptor

locus: BSU_03690

Molecular weight: 8.25 kDa

pI: 10.01

Protein length: 73 aa

Gene length: 222 bp

function: germination

product: negative effector of the [protein|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[protein|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]-[protein|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC] germinant receptor

essential: no

synonyms: gerKD, yczF

List of homologs in different organisms

Gene

Coordinates: 419,763 → 419,984

Phenotypes of a mutant

• faster [wiki|germination] with the AGFK germinant mixture [Pubmed|23625846]
• higher sensitivity to AGFK germinants ([wiki|germination] at lower concentrations) [Pubmed|23625846]

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The protein

Structure

• [AF|O31479]

[wiki|Localization]

• membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|DBC4A2711F5079AF1C1B743DA8D7A813DAA0827F|gerKD]

  description: [Pubmed|22383849]

  regulation

  • expressed during [wiki|sporulation] in the forespore ([protein|search|SigG]) [Pubmed|23625846]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|23625846], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• BKE03690 (Δ[gene|DBC4A2711F5079AF1C1B743DA8D7A813DAA0827F|gerKD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTTCATCATCCTCCGC,  downstream forward: _UP4_AACCCCTAAAGGGGGGCGCT
• BKK03690 (Δ[gene|DBC4A2711F5079AF1C1B743DA8D7A813DAA0827F|gerKD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCTTCATCATCCTCCGC,  downstream forward: _UP4_AACCCCTAAAGGGGGGCGCT

References

• 23625846,22383849,27766092