glgB

168

1,4-alpha-glucan branching enzyme

locus: BSU_30980

Molecular weight: 73.49 kDa

pI: 6.24

Protein length: 627 aa

Gene length: 1884 bp

function: glycogen biosynthesis

product: 1,4-alpha-glucan branching enzyme

essential: no

ec: 2.4.1.18

synonyms: glgB

List of homologs in different organisms, belongs to COG0296 (Galperin et al., 2021)

Gene

Coordinates: 3,169,763  3,171,646

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The protein

Catalyzed reaction/ biological activity

• Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain (according to UniProt)

Protein family

• [wiki|glycosyl hydrolase 13 family] (according to UniProt)

Structure

• [PDB|5GQU] (from Cyanothece sp., 46% identity) [pubmed|28193843]
• [AF|P39118]

Paralogous protein(s)

• [protein|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]

Expression and Regulation

Operons

• genes: [gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]-[gene|B665735B76462724A3C7A1E89D3E613C18F21399|glgC]-[gene|63A4FD35A2E72C665BFA3F2632F06C7A0C954087|glgD]-[gene|EBEA85AD543B79C321CB5F28D1B7E1248640DC1D|glgA]-[gene|3AB4513126EFF54996A54CB7DEB6062C1A06AAC3|glgP]

  description: [Pubmed|8145641]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|8145641]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8145641], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• BKE30980 ([gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAATCATCCTTTCTGA,  downstream forward: _UP4_AAAAAAAGAGGAGAGATAAA
• BKK30980 ([gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAATCATCCTTTCTGA,  downstream forward: _UP4_AAAAAAAGAGGAGAGATAAA

References

• 8145641,28193843