gpr

168

germination protease (degradation of SASPs)

locus: BSU_25540

Molecular weight: 40.12 kDa

pI: 5.2

Protein length: 368 aa

Gene length: 1107 bp

function: degradation of SASPs

product: germination protease (degradation of SASPs)

essential: no

synonyms: gpr

List of homologs in different organisms, belongs to COG5911 (Galperin et al., 2021)

Gene

Coordinates: 2,634,505  2,635,611

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The protein

Catalyzed reaction/ biological activity

• degradation of small, acid-soluble spore proteins (SASPs) [Pubmed|8478323]
• Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala (according to UniProt)

Protein family

• peptidase A25 family (single member, according to UniProt)

Structure

• [PDB|1C8B] (from B. megaterium, 68% identity) [pubmed|10864493]
• [AF|P22322]

[wiki|Localization]

• inner spore membrane [Pubmed|26731423]

Expression and Regulation

Operons

• genes: [gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]-[gene|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP]

  description: [Pubmed|16497325]

  regulation

  • expressed during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF], [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG], [wiki|SpoVT]) [Pubmed|16497325,1840582]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, [Pubmed|16497325], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325,1840582], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|1840582], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• BKE25540 ([gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC,  downstream forward: _UP4_TAAACAGTTCTACTTCCTCT
• BKK25540 ([gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC,  downstream forward: _UP4_TAAACAGTTCTACTTCCTCT

References

• 16199582,9045848,8188581,8071242,1732215,16497325,7961468,8071212,9748439,1840582,7608092,8478323,23927687,26731423,10864493,35638784