gsiB

168

general stress protein, prevents enzyme inactivation upon freeze-thaw treatments

locus: BSU_04400

Molecular weight: 13.66 kDa

pI: 5.14

Protein length: 123 aa

Gene length: 372 bp

function: response to water deficits

product: general stress protein

essential: no

synonyms: gsiB

List of homologs in different organisms, belongs to COG3729 (Galperin et al., 2021)

Gene

Coordinates: 494,506 → 494,877

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The protein

Structure

• [PDB|3JQH] (human glycan binding receptor, 31% identity) [pubmed|19835887]
• [AF|P26907]

Expression and Regulation

Operons

• genes: [gene|FE56282883B3229D3D6836A1BE7EA4292D3CC799|gsiB]

  description: [Pubmed|1378051]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|11544224]

  sigma factors

  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [Pubmed|11157964], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• 1A735 ( ''gsiB''::''kan''), [Pubmed|1378051], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A735&Search=1A735 BGSC]
• BKE04400 (Δ[gene|FE56282883B3229D3D6836A1BE7EA4292D3CC799|gsiB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTGAATTCCTCCTTTA,  downstream forward: _UP4_TAATCAAAAGCATAAAGGCA
• BKK04400 (Δ[gene|FE56282883B3229D3D6836A1BE7EA4292D3CC799|gsiB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTGAATTCCTCCTTTA,  downstream forward: _UP4_TAATCAAAAGCATAAAGGCA

References

• 9669336,12884008,7651322,11544224,1378051,21421783,23861025,11918677,19835887,29914988