hemB

168

porphobilinogen synthase

locus: BSU_28130

Molecular weight: 36.05 kDa

pI: 4.98

Protein length: 324 aa

Gene length: 975 bp

function: heme biosynthesis

product: porphobilinogen synthase

essential: no

ec: 4.2.1.24

synonyms: hemB

List of homologs in different organisms, belongs to COG0113 (Galperin et al., 2021)

Gene

Coordinates: 2,874,202  2,875,176

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The protein

Catalyzed reaction/ biological activity

• 2 5-aminolevulinate --> porphobilinogen + 2 H₂O (according to UniProt)

Protein family

• ALAD family (single member, according to UniProt)

[wiki|Cofactors]

• zinc

Structure

• [PDB|1W5Q] (from ''Pseudomonas aeruginosa'', 48% identity) [Pubmed|15644204]
• [AF|P30950]

Modification

• phosphorylated on Arg-217 [Pubmed|22517742]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|B1413EB525000744BE8E429E760220A13BC4530D|hemA]-[gene|88616BA1E853E11BE0CB18F06287CA4C68143148|hemX]-[gene|D266E22E07B849E2AEB0EF232C49A2FE1EA4E02B|hemC]-[gene|33459D4389F85D60311FCE770BBA0ED9802BA342|hemD]-[gene|70263A55987F110209367CF45AD65A584AD3077D|hemB]-[gene|A54B2C86638D474F5F892003B42C535B5B39A42E|hemL]

  description: [Pubmed|1672867]

  regulation

  • induced by hydrogen peroxide ([protein|search|PerR]) [Pubmed|11532148]

  regulatory mechanism

  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]: repression, [Pubmed|11532148], in [regulon|protein:00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1672867], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE28130 ([gene|70263A55987F110209367CF45AD65A584AD3077D|hemB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCGGTGTCTATTAAATGATT,  downstream forward: _UP4_TAATTTTATTCAGTTGACAG
• BKK28130 ([gene|70263A55987F110209367CF45AD65A584AD3077D|hemB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCGGTGTCTATTAAATGATT,  downstream forward: _UP4_TAATTTTATTCAGTTGACAG

References

Reviews

• 28123057

Original Publications

• 10217486,11532148,1672867,4214010,804803,12926268,20823524,22517742,15644204