iolX

168

scyllo-inositol 2-dehydrogenase

locus: BSU_10850

Molecular weight: 37.33 kDa

pI: 5.3

Protein length: 342 aa

Gene length: 1029 bp

function: utilization of scyllo-inositol

product: scyllo-inositol 2-dehydrogenase

essential: no

synonyms: iolX, yuxD, yucG, yisS

List of homologs in different organisms, belongs to COG0673 (Galperin et al., 2021)

Gene

Coordinates: 1,164,370  1,165,398

Phenotypes of a mutant

• impaired growth with scyllo-inositol as the only carbon source [Pubmed|20133360]

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The protein

Catalyzed reaction/ biological activity

• NAD+ + scyllo-inositol --> H⁺ + NADH + scyllo-inosose (according to UniProt)

Protein family

• [wiki|Gfo/Idh/MocA family] (according to UniProt)

[wiki|Cofactors]

• NAD⁺ [pubmed|28693424]

Structure

• [PDB|3EZY] (from Thermotoga maritima, 37% identity)
• [AF|P40332]

Paralogous protein(s)

• [protein|03BE6DA854612B3F6741E0E167AB310F0DE96285|yfiI], [protein|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC], [protein|484D17480DFAD3667E0EBC6D38854A7546A8DB46|yteT], [protein|920C5750CB95102B073C32874D512E8D636D2C7D|iolU], [protein|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG], [protein|942BAE9FA023DFCA06B6D26A856A72F2979E23FA|yrbE], [protein|7DFE74C751A67CCC84579D52005E1399B0A10166|iolW]

Expression and Regulation

Operons

• genes: [gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX]

  description: [pubmed|22383849]

  regulation: induced by scyllo-inositol [pubmed|28693424]

  regulatory mechanism

  • [protein|17F5188958A415D8D09439E02E1D1E0661B5C760|iolQ]: repression, [pubmed|28693424], in [regulon|protein:17F5188958A415D8D09439E02E1D1E0661B5C760|iolQ regulon]

Biological materials

Mutant

• MGNA-B191 (yisS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1190 NBRP B. subtilis, Japan]
• BKE10850 ([gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10850 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTCCCATCCTCTCCTTT,  downstream forward: _UP4_TAAAAGCTAAGTATGTTGCA
• BKK10850 ([gene|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|iolX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10850 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTCCCATCCTCTCCTTT,  downstream forward: _UP4_TAAAAGCTAAGTATGTTGCA

References

• 20133360,28693424