ispG

168

similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis

locus: BSU_25070

Molecular weight: 40.42 kDa

pI: 6.8

Protein length: 377 aa

Gene length: 1134 bp

function: MEP pathway of isoprenoid biosynthesis

product: 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase

essential: yes

ec: 1.17.7.3

synonyms: ispG, yqfY

List of homologs in different organisms, belongs to COG0821 (Galperin et al., 2021)

Gene

Coordinates: 2,589,123  2,590,256

Phenotypes of a mutant

• essential [Pubmed|12682299]

Advertisement

The protein

Catalyzed reaction/ biological activity

• (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H⁺ + H₂O + oxidized [flavodoxin] --> 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin] (according to UniProt)

Protein family

• ispG family (single member, according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|3NOY] (from ''Aquifex aeolicus'', 51% identity) [Pubmed|20932974]
• [AF|P54482]

[wiki|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|yqfZ]-[gene|4992F5C2ED7DE37C08D5715F7E8851F5FDB51059|ispG]

  description: [Pubmed|12662922]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|12662922,15383836]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

References

• 12662922,15383836,17458547,23832336,23840410,20932974