licC

168

lichenan-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIC of the [category|SW.1.2.2|PTS]

locus: BSU_38580

Molecular weight: 48.37 kDa

pI: 8.58

Protein length: 452 aa

Gene length: 1359 bp

function: lichenan uptake and phosphorylation

product: lichenan-specific lichenan-specific [category|SW.1.2.2|PTS], EIIC component

essential: no

synonyms: licC, celB

List of homologs in different organisms, belongs to COG1455 (Galperin et al., 2021)

Gene

Coordinates: 3,960,192  3,961,550

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The protein

Protein family

• [category|SW.1.2.2|PTS] permease, lactose family [Pubmed|10627040]

[wiki|Domains]

• [wiki|PTS EIIC domain] type-3 (aa 8-421) (according to UniProt)

Structure

• [PDB|3QNQ] (EIIC component of diacetylchitobiose specific PTS from ''Bacillus cereus''; 60% identity, 87% similarity) [Pubmed|21471968]
• [AF|P46317]

Paralogous protein(s)

• [protein|0650C9EAB395EB6ABDADBE72F0DA9F8E5E606B0E|ywbA], [protein|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]-[gene|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC]-[gene|E8B6C8E722BEE866878CDD816B7828C132F94FCD|licA]-[gene|84D8AA0AFBF46273F6B4198B49440D55470FEC0F|licH]

  description: [Pubmed|8990303]

  regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|8990303]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|8990303], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|licR]: activation, [Pubmed|8990303], in [regulon|protein:E087ABA705CE94E2CAD44A0F2FB265B0F7B04399|licR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8990303], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE38580 ([gene|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACATAGCCCGCCCCTCTCT,  downstream forward: _UP4_CTGTAGAGAAAGGAAGTGAA
• BKK38580 ([gene|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACATAGCCCGCCCCTCTCT,  downstream forward: _UP4_CTGTAGAGAAAGGAAGTGAA

References

• 19087206,8990303,16872404,10438772,10627040,8990303