lspA

168

signal peptidase II

locus: BSU_15450

Molecular weight: 17.28 kDa

pI: 9.13

Protein length: 154 aa

Gene length: 465 bp

function: protein secretion

product: signal peptidase II

essential: no

ec: 3.4.23.36

synonyms: lspA, lsp

List of homologs in different organisms, belongs to COG0597 (Galperin et al., 2021)

Gene

Coordinates: 1,616,744  1,617,208

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The protein

Catalyzed reaction/ biological activity

• Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains (according to UniProt)

Protein family

• peptidase A8 family (single member, according to UniProt)

Structure

• [PDB|5DIR] (from Pseudomonas aeruginosa, 38% identity), [pubmed|26912896]
• [AF|Q45479]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]-[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]-[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE15450 ([gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAAACGTTCCTCCAGTTT,  downstream forward: _UP4_GGGAAAAAGAAAAAGGAGCA
• BKK15450 ([gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAAACGTTCCTCCAGTTT,  downstream forward: _UP4_GGGAAAAAGAAAAAGGAGCA

References

Reviews

• 34410368

Original Publications

• 9882689,10497172,9880550,26912896