lytS

168

two-component sensor kinase, control of pyruvate utilization

locus: BSU_28930

Molecular weight: 64.68 kDa

pI: 8.24

Protein length: 593 aa

Gene length: 1782 bp

function: control of pyruvate utilization

product: two-component sensor kinase

essential: no

synonyms: lytS

List of homologs in different organisms, belongs to COG3275 (Galperin et al., 2021)

Gene

Coordinates: 2,956,486  2,958,267

Phenotypes of a mutant

• no growth with pyruvate as the single carbon source [Pubmed|27422364]

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The protein

Catalyzed reaction/ biological activity

• ATP + [protein|search|L-histidine] --> ADP + [protein|search|N-phospho-L-histidine] (according to UniProt)

[wiki|Domains]

• 5 transmembrane regions
• [wiki|GAF domain] (aa 238-363) (according to UniProt)
• [wiki|Histidine kinase domain] (aa 363-580) (according to UniProt)

Structure

• [AF|P94513]

Modification

• autophosphorylation on a His residue

[wiki|Localization]

• membrane (putative, due to presence of 5 membrane-spanning domains, according to Uniprot)

Expression and Regulation

Operons

• genes: [gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]-[gene|53BA24E91EEC797C95B8975C487F3C50CBBFA2E3|lytT]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A015 (lytS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/15 NBRP B. subtilis, Japan]
• BKE28930 ([gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCATGTCACCTGTTCTT,  downstream forward: _UP4_GAACATGCTCAGGGTGTTAA
• BKK28930 ([gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCATGTCACCTGTTCTT,  downstream forward: _UP4_GAACATGCTCAGGGTGTTAA

References

Reviews

• 29208748,29354650

Original Publications

• 10094672,27422364,28974613