mcsA

168

modulator of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-dependent repression, [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA] activates kinase activity of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]

locus: BSU_00840

Molecular weight: 20.88 kDa

pI: 6.62

Protein length: 185 aa

Gene length: 558 bp

function: control of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR] activity

product: activator of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] kinase activity

essential: no

synonyms: mcsA, yacH

List of homologs in different organisms, belongs to COG3880 (Galperin et al., 2021)

Gene

Coordinates: 101,927 → 102,484

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The protein

[wiki|Domains]

• [wiki|UVR domain] (aa 139-174) (according to UniProt)

Structure

• [AF|P37569]

Modification

• phosphorylated on Arg-169 [Pubmed|22517742]

Expression and Regulation

Operons

• genes: [gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]-[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[gene|151F226370D225776F3FE7EA4901485095F1AC45|radA]-[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]

  description: [Pubmed|8793870]

  regulation

  • expressed during germination and spore outgrowth [Pubmed|24244006]

  regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]: repression, [pubmed|9987115,11179229,16163393,17380125], in [regulon|protein:908DB17A39D518E84977250C55825E77FA02E391|ctsR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [pubmed|30962353], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8793870], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [pubmed|8793870] [pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

Biological materials

Mutant

• MGNA-B929 (yacH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1928 NBRP B. subtilis, Japan]
• ''mcsA::aphA3'' available from the Gerth lab
• BKE00840 (Δ[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT,  downstream forward: _UP4_GATAGTGAGGAGGAACAGGA
• BKK00840 (Δ[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT,  downstream forward: _UP4_GATAGTGAGGAGGAACAGGA

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

Antibody

• available in Gerth lab

References

• 12923084,8793870,11179229,9987115,16497325,19226326,9987115,11544224,22517742