med

168

lipoprotein, positive regulator of [gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK], stimulates [protein|511E71BB1981758857854C8E9BF657287CE60C11|kinD]-dependent phosphorylation of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]

locus: BSU_11300

Molecular weight: 35.16 kDa

pI: 4.6

Protein length: 317 aa

Gene length: 954 bp

function: regulation of competence

product: positive regulator of [gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]

essential: no

synonyms: med, yjaW

List of homologs in different organisms, belongs to COG1744 (Galperin et al., 2021)

Gene

Coordinates: 1,206,629  1,207,582

Phenotypes of a mutant

• impaired biofilm formation [Pubmed|21622736]

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The protein

Catalyzed reaction/ biological activity

• stimulates [protein|511E71BB1981758857854C8E9BF657287CE60C11|kinD]-dependent phosphorylation of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] [Pubmed|21622736]

Protein family

• BMP lipoprotein family (with [protein|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN], according to UniProt)

Structure

• [PDB|2HQB] (from B. halodurans, 49% identity) [pubmed|17636568]
• [AF|O32436]

[wiki|Localization]

• cell surface [Pubmed|12036071]

Expression and Regulation

Operons

• genes: [gene|D08B0A131374D2949ABC3EF8D6117C6BC6D94FE5|yjaV]-[gene|4DAB7847EA3848A34432C80124464543FA3DCA0F|med]-[gene|8914DE1312FD3C28E7D451A034814D92D1312B10|comZ]

  description: [pubmed|22383849]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

• genes: [gene|4DAB7847EA3848A34432C80124464543FA3DCA0F|med]-[gene|8914DE1312FD3C28E7D451A034814D92D1312B10|comZ]

  description: [Pubmed|9335269]

  regulation

  • repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9335269], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|2BF1CA62F5B8B54A25B70721D39B5BD38C0203EB|yjaU]-[gene|D08B0A131374D2949ABC3EF8D6117C6BC6D94FE5|yjaV]-[gene|4DAB7847EA3848A34432C80124464543FA3DCA0F|med]-[gene|8914DE1312FD3C28E7D451A034814D92D1312B10|comZ]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE11300 ([gene|4DAB7847EA3848A34432C80124464543FA3DCA0F|med]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTCATGTTCAACTCCTT,  downstream forward: _UP4_AACGAGTAATGGAGTGACGT
• BKK11300 ([gene|4DAB7847EA3848A34432C80124464543FA3DCA0F|med]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTCATGTTCAACTCCTT,  downstream forward: _UP4_AACGAGTAATGGAGTGACGT

References

• 9335269,12036071,14651647,17636568