murJ
168
lipid II flippase
locus
BSU_30050
Molecular weight
59.30 kDa
pI
10.09
function
export of lipid II
product
lipid II flippase
essential
no
synonyms
murJ, ytgP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5841 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,073,531 3,075,165
Phenotypes of a mutant
an ''[gene|12DB5AF311932991B66716BBCD08DA2C45B43BB9|amj] [gene|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]'' double mutant is not viable [Pubmed|25918422]
a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM] [gene|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]'' double mutant is not viable (due to the lack of both lipid II flippases) [Pubmed|25918422]
mutants lacking [protein|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ] produce long cells and chains of cells [Pubmed|19648239]
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The protein
Protein family
[wiki|Polysaccharide synthase family] (according to UniProt)
[wiki|MOP exporter family]
Structure
[PDB|5T77] (from ''Thermosipho africanus'', 20% identity) [Pubmed|28024149]
[AF|O34674]
Paralogous protein(s)
[protein|34A5CD28A3DBCB10B4E1C82E50143FA73B553919|ykvU], [protein|9633C40190B7D0EA264270FD44567C61A012AD15|spoVB], [protein|D6B1837075ECBEE1FEAC704DDB6795336092897F|yabM]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]-[gene|69052B24B577ABA4B20E610F679154DC97EFD8DE|ytzG]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A012 (ytgP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/12 NBRP B. subtilis, Japan]
BKE30050 ([gene|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCTTCATCACCATT, downstream forward: _UP4_TGATGAACTAGATATGATGA
BKK30050 ([gene|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCTTCATCACCATT, downstream forward: _UP4_TGATGAACTAGATATGATGA
References
Reviews
Original Publications
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Time of last update: 2024-04-24 20:16:37
Author of last update: Jstuelk