nadB

168

L-aspartate oxidase

locus: BSU_27870

Molecular weight: 58.07 kDa

pI: 6.45

Protein length: 531 aa

Gene length: 1596 bp

function: NAD biosynthesis

product: L-aspartate oxidase

essential: no

ec: 1.4.3.16

synonyms: nadB

List of homologs in different organisms, belongs to COG0029 (Galperin et al., 2021)

Gene

Coordinates: 2,847,871 → 2,849,466

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The protein

Catalyzed reaction/ biological activity

• L-aspartate + O₂ --> H₂O₂ + iminosuccinate (according to UniProt)

Protein family

• FAD-dependent oxidoreductase 2 family (with [protein|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA], according to UniProt)

[wiki|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|5KXJ] (from Salmonella typhimurium, 38% identity)
• [AF|P38032]

Modification

• phosphorylated on Arg-104 [Pubmed|22517742]

Biological materials

Mutant

• BKE27870 (Δ[gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGCCATCCTCCTGTTG,  downstream forward: _UP4_AGAAAAAACGAGGGGATTTG
• BKK27870 (Δ[gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGCCATCCTCCTGTTG,  downstream forward: _UP4_AGAAAAAACGAGGGGATTTG

labs

• [wiki|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]

References

• 8444804,18959769,16199587,20525796,22517742