nagA

168

N-acetylglucosamine-6-phosphate deacetylase

locus: BSU_35010

Molecular weight: 42.46 kDa

pI: 5.28

Protein length: 396 aa

Gene length: 1191 bp

function: N-acetylglucosamine utilization

product: N-acetylglucosamine-6-phosphate deacetylase

essential: no

ec: 3.5.1.25

synonyms: nagA

List of homologs in different organisms, belongs to COG1820 (Galperin et al., 2021)

Gene

Coordinates: 3,595,356  3,596,546

Phenotypes of a mutant

• no growth on N-acetylglucosamine [Pubmed|23667565]

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The protein

Catalyzed reaction/ biological activity

• H₂O + N-acetyl-D-glucosamine 6-phosphate --> acetate + D-glucosamine 6-phosphate (according to UniProt)

Protein family

• [wiki|Metallo-dependent hydrolases superfamily] (according to UniProt)

Structure

• [PDB|2VHL] [Pubmed|14557261]
• [AF|O34450]

Kinetic information

• K(M): 1.4 mM [Pubmed|14343123]

Expression and Regulation

Operons

• genes: [gene|8F4D075C9B9EF995E361F9BA5E8B52F42059C422|nagA]-[gene|0C8D7EAC2656E989E45B5B7E42FAA6D258956B56|nagB]-[gene|6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|nagR]

  description: [Pubmed|21602348]

  regulation

  • induced in the presence of N-acetylglucosamine ([protein|search|NagR]) [Pubmed|24673833,21602348,14343123]

  regulatory mechanism

  • [protein|6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|nagR]: repression, [Pubmed|24673833,21602348], in [regulon|protein:6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|nagR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23667565], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A337 (nagA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/337 NBRP B. subtilis, Japan]
• BKE35010 ([gene|8F4D075C9B9EF995E361F9BA5E8B52F42059C422|nagA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGATGATCCGCCTTTCT,  downstream forward: _UP4_ATATCCAAGGAGGCTGACCA
• BKK35010 ([gene|8F4D075C9B9EF995E361F9BA5E8B52F42059C422|nagA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGATGATCCGCCTTTCT,  downstream forward: _UP4_ATATCCAAGGAGGCTGACCA

References

Reviews

• 26159076

Original Publications

• 10627040,14343123,12850135,6174502,14557261,23667565,21602348,24673833,23876412,25564531