narG

narG
168

nitrate reductase (alpha subunit)

locus
BSU_37280
Molecular weight
138.86 kDa
pI
6.06
Protein length
Gene length
function
nitrate respiration, nitrogen assimilation
product
nitrate reductase (alpha subunit)
essential
no
synonyms
narG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5013 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,826,259 → 3,829,945
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The protein
Catalyzed reaction/ biological activity
quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
Protein family
[wiki|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)
[wiki|Domains]
[wiki|4Fe-4S Mo/W bis-MGD-type domain] (aa 47-111) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [Pubmed|11289299]
MoCo [Pubmed|11289299]
Structure
[PDB|1Q16] (the [protein|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[protein|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[protein|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI] complex of E. coli, 52% identity) [pubmed|12910261]
[AF|P42175]
[wiki|Localization]
cell membrane [Pubmed|23880299]
Expression and Regulation
Operons
genes
[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
description
[Pubmed|8846791,16428414]
regulation
expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]
regulatory mechanism
[protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]: activation, [Pubmed|8846791,16428414], in [regulon|protein:7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]→[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]

2024-03-24 10:37:27

ghost

55

2df0c9c15bd640f1035afac39ab71391c14fde4b

655A38484A21CE64690D3913F055D0C46476DC9F

Biological materials
Mutant
BKE37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC,  downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
BKK37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC,  downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
References
Reviews
11289299,33705278
Original Publications
9352926,23880299,8846791,16428414,12910261

B992698A12EE9D17DCEF075F82396D5456D35A20

Page visits: 4999

Time of last update: 2024-03-28 06:15:09

Author of last update: Jstuelk