narH

narH
168

nitrate reductase (beta subunit)

locus
BSU_37270
Molecular weight
55.30 kDa
pI
5.11
Protein length
Gene length
function
nitrate respiration, nitrogen assimilation
product
nitrate reductase (beta subunit)
essential
no
synonyms
narH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1140 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,824,806  3,826,269
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The protein
Catalyzed reaction/ biological activity
quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
[wiki|Domains]
3 [wiki|4Fe-4S ferredoxin-type domain]s (aa 7-36, aa 172-203, aa 205-234) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [Pubmed|11289299]
Structure
[PDB|1Q16] (the [protein|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[protein|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[protein|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI] complex of E. coli, 59% identity) [pubmed|12910261]
[AF|P42176]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
description
[Pubmed|8846791,16428414]
regulation
expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]
regulatory mechanism
[protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]: activation, [Pubmed|8846791,16428414], in [regulon|protein:7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]→[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]

2024-03-24 10:37:27

ghost

55

2df0c9c15bd640f1035afac39ab71391c14fde4b

655A38484A21CE64690D3913F055D0C46476DC9F

Biological materials
Mutant
BKE37270 ([gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTCATGACCATACCGATTT,  downstream forward: _UP4_TAACTTTTGATAAATGAGGT
BKK37270 ([gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTCATGACCATACCGATTT,  downstream forward: _UP4_TAACTTTTGATAAATGAGGT
References
Reviews
11289299,33705278
Original Publications
9352926,8846791,16428414,12910261

6181C785427277C097B81D67A93213AEAF701FF4

Page visits: 2789

Time of last update: 2024-03-29 01:14:37

Author of last update: Jstuelk