natA

168

Na⁺ [wiki|ABC transporter ](export) (ATP-binding protein)

locus: BSU_02750

Molecular weight: 27.73 kDa

pI: 8.8

Protein length: 246 aa

Gene length: 741 bp

function: sodium export

product: Na⁺ [wiki|ABC transporter ](export) (ATP-binding protein)

essential: no

synonyms: natA

List of homologs in different organisms, belongs to COG4555 (Galperin et al., 2021)

Gene

Coordinates: 296,429 → 297,169

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The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + Na⁺ --> ADP + H⁺ + Na⁺ + phosphate (according to UniProt)

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 2-237) (according to UniProt)

Structure

• [PDB|1VPL] (from Thermotoga maritima, 38% identity)
• [AF|P46903]

[wiki|Localization]

• membrane associated (via [protein|A8ADA02BBB549866DE330040CF6D06ECC3B00099|natB]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|B019FD0CB6F76C03DA5E76ABD1B324BA5E1A6936|natA]-[gene|A8ADA02BBB549866DE330040CF6D06ECC3B00099|natB]

  description: [Pubmed|17322186]

  regulatory mechanism

  • [protein|D3F6337B94A633730E514B05CA852BE9AA3741B3|natR]: activation, [Pubmed|17322186], in [regulon|protein:D3F6337B94A633730E514B05CA852BE9AA3741B3|natR regulon]

Biological materials

Mutant

• BKE02750 (Δ[gene|B019FD0CB6F76C03DA5E76ABD1B324BA5E1A6936|natA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCACAATCTCCCTTAT,  downstream forward: _UP4_AAGCTTGTCAGGGGGATTTC
• BKK02750 (Δ[gene|B019FD0CB6F76C03DA5E76ABD1B324BA5E1A6936|natA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCACAATCTCCCTTAT,  downstream forward: _UP4_AAGCTTGTCAGGGGGATTTC

References

• 9106203,10092453,17322186