nudF

168

involved in isopentenol (isoprenoid) biosynthesis, has RNA pyrophosphohydrolase activity

locus: BSU_23610

Molecular weight: 20.84 kDa

pI: 4.59

Protein length: 185 aa

Gene length: 558 bp

function: isoprenoid biosynthesis

product: unknown

essential: no

ec: 3.6.1.13

synonyms: nudF, yqkG

List of homologs in different organisms, belongs to COG0494 (Galperin et al., 2021)

Gene

Coordinates: 2,458,509 → 2,459,066

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The protein

Catalyzed reaction/ biological activity

• ADP-D-ribose + H₂O --> AMP + D-ribose 5-phosphate + 2 H⁺ (according to UniProt)

Protein family

• [wiki|Nudix hydrolase] (according to UniProt)

[wiki|Domains]

• [wiki|Nudix hydrolase domain] (aa 40-171) (according to UniProt)

Structure

• [PDB|1V8I] (from ''Thermus thermophilus'', 38% identity) [Pubmed|15210687]
• [AF|P54570]

Expression and Regulation

Operons

• genes: [gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]-[gene|9D05B104000EED08FB62D6F362FC5E9C2A5354D9|yqxK]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C410 (yqkG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2408 NBRP B. subtilis, Japan]
• BKE23610 (Δ[gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCATCTCCCGTTC,  downstream forward: _UP4_AAAGAAGCGCTCCAAGCACA
• BKK23610 (Δ[gene|CF38BE04F04919BB304789E9B3FE489385E468E6|nudF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCATCTCCCGTTC,  downstream forward: _UP4_AAAGAAGCGCTCCAAGCACA

References

• 17693564,10542272,15210687,31740579,35721994