ogt

168

O6-methylguanine DNA alkyltransferase

locus: BSU_13540

Molecular weight: 18.61 kDa

pI: 7.09

Protein length: 165 aa

Gene length: 498 bp

function: DNA repair

product: O6-methylguanine DNA alkyltransferase

essential: no

ec: 2.1.1.63

synonyms: ogt, dat

List of homologs in different organisms, belongs to COG0350 (Galperin et al., 2021)

Gene

Coordinates: 1,421,426  1,421,923

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The protein

Catalyzed reaction/ biological activity

• 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] --> 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
• 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] --> thymidine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)

Protein family

• MGMT family (with [protein|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB], according to UniProt)

Structure

• [PDB|1SFE] (from ''E. coli'', 36% identity, 51% similarity) [Pubmed|8156986]
• [AF|P11742]

Biological materials

Mutant

• BKE13540 ([gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT,  downstream forward: _UP4_TAAAACATATGGCACGTTCC
• BKK13540 ([gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT,  downstream forward: _UP4_TAAAACATATGGCACGTTCC

References

• 2506524,2105461,9366274