pbpA

168

class B penicillin-binding protein PBP 2A, required for [wiki|cell wall synthesis] during cell elongation, works together with [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA] in the [wiki|elongasome] for lateral [wiki|cell wall synthesis] and control of cell diameter

locus: BSU_25000

Molecular weight: 79.96 kDa

pI: 9.57

Protein length: 716 aa

Gene length: 2151 bp

function: formation of a rod-shaped peptidoglycan cell wall, spore outgrowth

product: penicillin-binding protein PBP 2A

essential: no

synonyms: pbpA, yqgF

List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)

Gene

Coordinates: 2,581,771  2,583,921

Phenotypes of a mutant

• a [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA] [gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH] double mutant is not viable [pubmed|36993441]

Advertisement

The protein

Catalyzed reaction/ biological activity

• required for [wiki|cell wall synthesis] during cell elongation [Pubmed|12896990]
• driver of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] dynamics [Pubmed|21636744,21636745]
• transpeptidase activity [pubmed|33709487]

Protein family

• [wiki|transpeptidase family] (according to UniProt)

Structure

• [PDB|3VSK] (from Staphylococcus aureus, 45% identity) [pubmed|22846910]
• [AF|P54488]

Paralogous protein(s)

• [protein|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]
• during vegetative growth: evenly distributed along the membrane [pubmed|14731276]
• localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]

Expression and Regulation

Operons

• genes: [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]

  description: [Pubmed|21815947]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-C461 (yqgF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2459 NBRP B. subtilis, Japan]
• BKE25000 ([gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT,  downstream forward: _UP4_TAAAAAAAAACGCTCACATG
• BKK25000 ([gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT,  downstream forward: _UP4_TAAAAAAAAACGCTCACATG

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jeff Errington] lab

GFP fusion

• 3103  [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]::pSG5043 (cat P_xyl-gfpa-[gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]1-804) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

labs

• [wiki|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

Original Publications

• 9851991,9139922,12896990,18957862,20487272,21636744,21636745,21815947,23895585,22846910,14731276,31086310,32152588,34411374,36993441,38126750