pksB

168

involved in polyketide synthesis

locus: BSU_17090

Molecular weight: 25.76 kDa

pI: 6.59

Protein length: 225 aa

Gene length: 678 bp

function: polyketide synthesis

product: unknown

essential: no

synonyms: pksB

List of homologs in different organisms, belongs to COG0491 (Galperin et al., 2021)

Gene

Coordinates: 1,782,713  1,783,390

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The protein

Protein family

• [wiki|metallo-beta-lactamase superfamily] (according to UniProt)

Structure

• [PDB|4YSB] (from Myxococcus xanthus, 28% identity) [pubmed|26082492]
• [AF|O34769]

Expression and Regulation

Operons

• genes: [gene|647B1D7E496E81E7FC7FD2BDCACC2486852BAC19|pksB]-[gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]

  description: [pubmed|22383849]

Biological materials

Mutant

• BKE17090 ([gene|647B1D7E496E81E7FC7FD2BDCACC2486852BAC19|pksB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTATACCATCCCTATCA,  downstream forward: _UP4_TAATCCTTCTGAATCTATTA
• BKK17090 ([gene|647B1D7E496E81E7FC7FD2BDCACC2486852BAC19|pksB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTATACCATCCCTATCA,  downstream forward: _UP4_TAATCCTTCTGAATCTATTA

References

Research papers

• 26082492