polC

168

DNA polymerase III, leading strand polymerase (alpha subunit), part of the [wiki|replisome]

locus: BSU_16580

Molecular weight: 162.42 kDa

pI: 5.14

Protein length: 1437 aa

Gene length: 4311 bp

function: [category|SW.3.1.1|DNA replication]

product: DNA polymerase III (alpha subunit)

essential: yes

ec: 2.7.7.7

synonyms: polC, mutI, dnaF, dnaP

List of homologs in different organisms, belongs to COG2176 (Galperin et al., 2021)

Gene

Coordinates: 1,727,133 → 1,731,446

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• 2'-deoxyribonucleoside 5'-triphosphate + DNA_(n) --> diphosphate + DNA_(n+1) (according to UniProt)
• required for bacteriophage SPP1 replication [Pubmed|23268446]

Protein family

• DNA polymerase type-C family (with [protein|48BC0FA9901F174472EB070C539EC75EC7C10E88|yorL] and [protein|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|dnaE], according to UniProt)

[wiki|Domains]

• [wiki|Exonuclease domain] (aa 421-578) (according to UniProt)

Structure

• [PDB|3F2B] (from ''Geobacillus kaustophilus'', 71% identity) [Pubmed|19106298]
• [AF|P13267]

[wiki|Localization]

• forms foci [Pubmed|21958350]
• about three molecules of PolC at each replication fork [pubmed|28002733]

Expression and Regulation

Operons

• genes: [gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]

  description: [Pubmed|22383849]

• genes: [gene|4A637EB9B400D30E5C6246D1E0EB5E6A30F38A16|uppS]-[gene|7E7F6BBA03C443A5AFA05A6D9352FEF1212A5AF6|cdsA]-[gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]

  description: [Pubmed|22383849]

additional information

• about 60 molecules per cell [pubmed|28002733]

References

Reviews

• 19106294,28073967,29197672

Original Publications

• 15186423,20122408,11721055,23563155,23268446,21740522,21958350,24062730,24106089,19106298,28002733,28575448,28878042,30686488,31251806,36574412