PolY1

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  • Description: translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis, promotes efficient replisome progression through lagging-strand genes, thereby reducing potentially detrimental breaks and single-stranded DNA at these loci

Gene name polY1
Synonyms yqjH
Essential no
Product translesion synthesis (TLS-) DNA polymerase Y1
Function generation of mutations in stationary phase
Gene expression levels in SubtiExpress: polY1
Interactions involving this protein in SubtInteract: PolY1
Metabolic function and regulation of this protein in SubtiPathways:
polY1
MW, pI 46 kDa, 9.953
Gene length, protein length 1242 bp, 414 aa
Immediate neighbours gndA, mifM
Sequences Protein DNA DNA_with_flanks
Genetic context
YqjH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PolY1 expression.png















Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU23870

Phenotypes of a mutant

reduced UV-induced mutagenesis PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: constitutively expressed PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1111 (spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Samuel Million-Weaver, Ariana N Samadpour, Daniela A Moreno-Habel, Patrick Nugent, Mitchell J Brittnacher, Eli Weiss, Hillary S Hayden, Samuel I Miller, Ivan Liachko, Houra Merrikh
An underlying mechanism for the increased mutagenesis of lagging-strand genes in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2015, 112(10);E1096-105
[PubMed:25713353] [WorldCat.org] [DOI] (I p)

Richard T Pomerantz, Isabel Kurth, Myron F Goodman, Mike E O'Donnell
Preferential D-loop extension by a translesion DNA polymerase underlies error-prone recombination.
Nat Struct Mol Biol: 2013, 20(6);748-55
[PubMed:23686288] [WorldCat.org] [DOI] (I p)

Andrea M Rivas-Castillo, Ronald E Yasbin, E Robleto, Wayne L Nicholson, Mario Pedraza-Reyes
Role of the Y-family DNA polymerases YqjH and YqjW in protecting sporulating Bacillus subtilis cells from DNA damage.
Curr Microbiol: 2010, 60(4);263-7
[PubMed:19924481] [WorldCat.org] [DOI] (I p)

Stéphane Duigou, S Dusko Ehrlich, Philippe Noirot, Marie-Françoise Noirot-Gros
DNA polymerase I acts in translesion synthesis mediated by the Y-polymerases in Bacillus subtilis.
Mol Microbiol: 2005, 57(3);678-90
[PubMed:16045613] [WorldCat.org] [DOI] (P p)

Stéphane Duigou, S Dusko Ehrlich, Philippe Noirot, Marie-Françoise Noirot-Gros
Distinctive genetic features exhibited by the Y-family DNA polymerases in Bacillus subtilis.
Mol Microbiol: 2004, 54(2);439-51
[PubMed:15469515] [WorldCat.org] [DOI] (P p)

Huang-Mo Sung, Gabriel Yeamans, Christian A Ross, Ronald E Yasbin
Roles of YqjH and YqjW, homologs of the Escherichia coli UmuC/DinB or Y superfamily of DNA polymerases, in stationary-phase mutagenesis and UV-induced mutagenesis of Bacillus subtilis.
J Bacteriol: 2003, 185(7);2153-60
[PubMed:12644484] [WorldCat.org] [DOI] (P p)